Lysozyme fermentation method

Abstract

The invention discloses a lysozyme fermentation method. Step 1: Packing column, cation exchange resin CM-Sephdex is soaked in dilute buffered saline solution for a day and night, and gently stirred tobalance it, during the balance with the solvent, let The CM-Sephdex material of the column is settling, the small particles floating in the suspension are removed by the pouring method, and then thecolumn is packed. Note that the bubbles are not trapped when the column is packed, and the size of the column depends on the processing scale; Step 2: Collect the extract, The egg white is collected,and after filtering with double-layer gauze to remove the broken egg shell and the embryo rope, water is added twice as much as the volume thereof, and the mixture is carefully stirred and mixed, andit is necessary to pay attention to the technical field of lysozyme fermentation. The lysozyme fermentation method is a lysozyme product which is separated and dried by crystallization, and has insufficient purity, and is further purified by a recrystallization method. After the lysozyme-associated solute is dissolved in a solvent, the solvent is adsorbed, and the solute is subjected to solute. Recrystallization can be purified.

Description

technical field [0001] The invention relates to the technical field of lysozyme fermentation, in particular to a lysozyme fermentation method. Background technique [0002] Lysozyme, also known as cell wall hydrolase, can specifically act on the peptidoglycan molecule of bacterial cell wall, and can break the β-1,4 glycosidic bond between N-acetylmuramic acid and acetylglucosamine, making the bacterial cell wall have to relax to achieve the effect of dissolving bacteria. Lysozyme is widely distributed in higher animal and plant tissues and their secretions, protozoa, insects, plants and various microorganisms. As one of the non-specific immune factors in the normal body fluids and tissues of the human body, lysozyme has a variety of pharmacological effects, participates in various immune reactions of the body, has antibacterial and anti-viral effects, and plays a role in the normal defense function and non-specific immunity of the body. It plays an important role in mainta...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a fermentation method of lysozyme, which solves the problem that the existing egg white lysozyme cannot use the egg yolk liquid during production, so that the egg yolk is wasted in large quantities, and the production of the prior art The purity of egg white lysozyme is not high, which affects the quality of lysozyme