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Method for effectively improving rooting rate of hybrid tree tissue culture seedlings

A tissue culture seedling and rooting rate technology, applied in the biological field, can solve the problems of high production cost, low survival rate of tissue culture seedling transplanting, no rooting, etc. The effect of rooting rate

Pending Publication Date: 2019-01-18
CHUXIONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aseptic rooting induced by tissue culture of woody plants often causes no rooting, root callus and other phenomena, resulting in low survival rate of tissue culture seedlings transplanted and high production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1) use the rootless seedlings of more than 7 generations of replanting to take root, and the young shoot tip incision of the rootless seedlings is washed with sterile water, and inoculated after drying the water on sterile filter paper;

[0042] 2) cutting the young shoot tips of the rootless seedlings after aseptic filtration into 1 cm, and inoculating them in the proliferation medium for 2 days in the dark, and inducing;

[0043] 3) Step 2) is induced to carry out one-step rooting, and the improved WPM medium used in the one-step rooting is: an appropriate amount of improved WPM, sucrose 15g / L, NAA 0.1g / L, IBA 0.1g / L, ABT 0.1g / L, Agar 6g / L;

[0044] 4) After step 3) one-step rooting is completed, carry out dark treatment for 4 days, then carry out two-step rooting, and the liquid-solid medium used for two-step rooting is: perlite 15g / ml, liquid medium 55ml;

[0045] 5) The two-step rooting is carried out under light for 20 days, and new shoots and new roots grow from...

Embodiment 2

[0052] 1) use the rootless seedlings of more than 7 generations of replanting to take root, and the young shoot tip incision of the rootless seedlings is washed with sterile water, and inoculated after drying the water on sterile filter paper;

[0053] 2) cutting the young shoot tips of the rootless seedlings after aseptic filtration into 2 cm, and inoculating them in the proliferation medium for 5 days in the dark, and inducing;

[0054] 3) Step 2) is induced to carry out one-step rooting, and the improved WPM medium used in the one-step rooting is: an appropriate amount of improved WPM, sucrose 25g / L, NAA 0.5g / L, IBA 0.2g / L, ABT 0.2g / L, AC 0.1g / L, agar 7g / L;

[0055] 4) After step 3) one-step rooting is completed, carry out dark treatment for 6 days, then carry out two-step rooting, and the liquid-solid medium used for two-step rooting is: perlite 20g / ml, liquid medium 60ml;

[0056] 5) The two-step rooting is carried out under light for 30 days, and new shoots and new root...

Embodiment 3

[0063] 1) use the rootless seedlings of more than 7 generations of replanting to take root, and the young shoot tip incision of the rootless seedlings is washed with sterile water, and inoculated after drying the water on sterile filter paper;

[0064] 2) cutting the young shoot tips of the rootless seedlings after aseptic filtration into 1.5 cm, inoculating them in the proliferation medium for 3 days in the dark, and inducing;

[0065] 3) Step 2) is induced to carry out one-step rooting, and the improved WPM medium used in the one-step rooting is: an appropriate amount of improved WPM, sucrose 20g / L, NAA 0.3g / L, IBA 0.1g / L, ABT 0.1g / L, Agar 6.8g / L;

[0066] 4) After step 3) one-step rooting is completed, carry out dark treatment for 5 days, then carry out two-step rooting, and the liquid-solid medium used for two-step rooting is: perlite 18g / ml, liquid medium 58ml;

[0067] 5) The two-step rooting is carried out under light for 25 days, and the budded stem section in the liq...

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Abstract

The invention discloses a method for effectively improving the rooting rate of hybrid tree tissue culture seedlings. The method comprises the following steps: (1) enabling a rootless seedling which issubcultivated for at least seven generations to root; (2) cutting young stem tips of the rootless seedling after being sterilely filtered into a length of 1 to 2 cm, performing the dark treatment, and inducing; (3) performing primary rooting on the seedling induced in step (2); (4) after the primary rooting is completed in step (3), performing the dark treatment for 4 to 6 days, performing the secondary rooting, wherein a liquid-solid culture medium for the secondary rooting is prepared from 15 to 20 g / ml of perlite and 55 to 60 ml of liquid culture medium; and (5) performing the light illumination for 20 to 30 days after the secondary rooting. By adopting the method disclosed by the invention, the virus probability of the seed seedlings can be reduced; the cluster buds are induced at theproliferation phase, so that the proliferation times can be improved, and the proliferation times is greater than 6 to 8 times; the rooting rate of the tissue culture seedlings can be apparently improved, and the rooting rate is 97 percent; and the transplanting survival rate is 99 percent.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for effectively improving the rooting rate of hybrid tree tissue culture seedlings. Background technique [0002] Due to factors such as the longer physiological life of woody plants, more chromosome numbers, high cortex and xylem differentiation, and scientific research and education systems, a large number of research results carried out by many research institutes in my country are separated from the economic feasibility of commercial production. Various indicators such as stability and uniformity of seedlings cannot be applied to large-scale commercial production. Commercial activities for seedling propagation using tissue culture techniques in woody plants have only begun in recent years. [0003] The main technical reasons restricting the development of the woody plant group cultivation seedling industry are: explant xylem carrying bacteria, proliferation and callus, a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 邱璐
Owner CHUXIONG NORMAL UNIV
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