Method for screening high-secretion type material from tobacco mutants

Abstract

The invention discloses a method for screening a high-secretion type material from a tobacco mutant library. The method comprises the following steps of: step (1): seed treatment: step (2): preliminary screening of mutation groups: step (3): screening and identification of single plants; step (4): repeated identification (F2-generation identification); step (5): strain identification. The method disclosed by the invention has the beneficial effects that at the initial stage of identification, the density and the type of glandular hairs of a leaf surface are intuitively screened by utilizing the correlation of the density and the type of the glandular hairs and secreta of the leaf surface, and then on the basis, identification of the secreta of the leaf surface and the screening of the high-secretion type material are carried out, so that the screening result is accurate and reliable; at a seedling stage, 90% or more of mutant plants are eliminated, so that the planting of large-field materials is greatly reduced, and the cost for land and labor and the like is reduced; because of reduction of the planting materials, the identification quantity of the secreta at a mature stage is also reduced, so that the cost is greatly reduced, the screening time is shortened and the screening efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for screening high-secretion materials from tobacco mutants. Background technique [0002] Tobacco leaf exudates are closely related to the quality and resistance of tobacco leaves. Varieties with high content of leaf secretions have strong tobacco resistance, high tobacco leaf oil content, good quality, and better smoking quality. With the increasing attention to smoking and health issues, it is increasingly important to reduce tar and harm, and the quality requirements for tobacco leaves are also getting higher and higher. In order to improve the quality of tobacco leaves and increase the variety resistance, increasing the content of leaf secretions is a very effective way. [0003] The study found that for tobacco, the density and type of glandular trichomes are closely related to the content of leaf secretions. The denser the glandular trichomes, especially the secret...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems that the existing high-secretion tobacco material mainly adopts the method of planting all the mutagenized offspring and extracting and identifying leaf secretions one by one, the screening workload is heavy, the cost is high, and the efficiency is low. The purpose of the present invention is to provide a A method for screening high-secretion materials from tobacco mutants, which can greatly reduce the screening workload, save costs, and improve screening efficiency

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