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Static-pile composting method for treating cow dung harmlessly

A technology of harmless treatment and composting method, which is applied in the field of agricultural biology, can solve the problems of safety risks and weak microbial detection of beef, and achieve the effects of improving safety, low production cost and ensuring health

Inactive Publication Date: 2019-01-15
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the implementation of microbiological testing of beef in my country is relatively weak compared with developed countries, and there are safety risks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] S1. Preparation of microbial agents, Bacillus subtilis ABTNL-2, Bacillus licheniformis, and Bacillus cereus were inoculated in liquid medium A respectively, and cultured with shaking at 37°C for 24 hours to obtain three concentrations of 10 8 ~10 9 CFU mL -1 Bacillus bacteria liquid; inoculate lactic acid bacteria in liquid medium B, and culture it statically at 37°C for 72 hours to obtain a concentration of 10 8 ~10 9 CFU mL -1 of lactic acid bacteria. After mixing the above four bacterial solutions in proportion, a microbial composite bacterial agent for cow dung composting is obtained;

[0025] S2. Establishment of cow dung composting. Mix cow dung and plant materials according to the weight ratio of 1:0.05-0.1, inoculate the microbial compound bacterial agent in S1, mix evenly, and adjust the compost to a moisture content of 55-65% before establishment For composting, use a 20-30cm thick haystack to cover the top, bottom and surroundings of the compost to keep ...

Embodiment 1

[0040] S1, the preparation of microbial bacterial agent

[0041] S11, preparation of bacillus bacterium liquid: respectively inoculate Bacillus subtilis ABTNL-2, Bacillus licheniformis, Bacillus cereus in liquid culture medium A (beef extract 4g, peptone 4g, glucose 4g, starch 3g, NaCl 7g, distilled water 1000mL , pH7.0), cultured by shaking at 37°C for 24 hours to obtain the bacterial liquid, the corresponding volumes of the bacterial liquid for the three kinds of bacteria were 1.5L, 0.9L and 1.2L, and the concentrations were 8×10 8 CFU mL -1 , 2×10 8 CFU mL -1 and 5×10 8 CFU mL -1 ;

[0042] S12, preparation of lactic acid bacteria liquid: inoculate lactic acid bacteria in liquid medium B (peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, Tween 80 1mL, sodium acetate 4g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.5g, sulfuric acid Manganese 0.2g, distilled water 1000mL, pH 6.5), culture at 37°C for 72h, and obtain a concentration of 3×10 8 CFU ...

Embodiment 2

[0047] S1, the preparation of microbial bacterial agent

[0048] S11, preparation of bacillus bacterium liquid: inoculate Bacillus subtilis ABTNL-2, Bacillus licheniformis, Bacillus cereus in liquid medium A (beef extract 3g, peptone 3.5g, glucose 3g, starch 2g, NaCl 6g, distilled water respectively) 1000mL, pH7.2), shake culture at 37°C for 36h to obtain the bacterial liquid, the concentration of the three bacterial liquids is 10 8 CFU mL -1 ;

[0049] S12, preparation of lactic acid bacteria liquid: inoculate lactic acid bacteria in liquid medium B (peptone 8g, beef extract 8g, yeast extract 4g, glucose 18g, Tween 80 0.9mL, sodium acetate 5g, dipotassium hydrogen phosphate 1.8g, magnesium sulfate 0.6g , manganese sulfate 0.3g, distilled water 1000mL, pH 6.2), at 37 ℃ for 56h, the obtained concentration is 10 8 CFU mL -1 lactic acid bacteria

[0050] S13 Mixing: Mix four bacterial liquids of Bacillus subtilis ABTNL-02 obtained in step S11, Bacillus licheniformis, Bacillu...

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Abstract

The invention discloses a static-pile composting method for treating cow dung harmlessly. The method comprises the three steps that S1, a microbial inoculant is prepared, in specific, four kinds of microorganisms including ABTNL-2 bacillus subtilis, bacillus licheniformis, bacillus cereus and lactobacillus are utilized to prepare the microbial complex inoculant for cow dung composting; S2, cow dung composting is built, in specific, the microbial inoculant is inoculated to the mixture after the cow dung and plant materials are mixed, composting is built up after the materials are mixed evenly,a ventilation and temperature monitoring device is arranged in the compost, and the surrounding part of the pile body is completely covered with stack to preserve the heat; and S3, compost managementis conducted, in specific, air is injected into the pile body so as to ensure rapid warming and decomposition of the compost, the pile body temperature is monitored, the product after composting serves as biological organic fertilizer. The method can achieve rapid, simple and convenient harmless treatment of cow dung of small-scale farmers, and particularly can rapidly and completely inactivate the enterohemorrhagic escherichia coli O157:H7 in the cow dung, and the composting product can serve as the biological organic fertilizer to achieve resource utilization.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology, and in particular relates to a static composting method for harmlessly treating cow dung. Background technique [0002] Beef is one of the main sources of meat in my country. With the improvement of people's living standards, the proportion of beef consumption has increased year by year, and the food safety problems that follow have become increasingly prominent. Beef is one of the main foods that cause enterohemorrhagic E. coli (enterohemorrhage E. Coli, EHEC) O157: H7 bacterial food poisoning. Eating beef contaminated with EHECO157:H7 can cause not only vomiting, diarrhea and other intestinal diseases, but also complications such as hemolytic uremic syndrome and encephalopathy, and even death. Therefore, it is called an emerging infectious disease together with AIDS and Ebola hemorrhagic fever, and is highly valued by the World Health Organization. [0003] The contamination of E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F15/00C05F17/00
CPCC05F3/00C05F17/00C05F11/00C05F11/08Y02W30/40
Inventor 王丽丽徐永平李晓宇陈红莉侯扶琴吴妍妍续彦龙
Owner DALIAN UNIV OF TECH
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