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Enzyme dilution liquid and preparation method thereof

A diluent and purified water technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of decreased immune activity, reduce enzyme denaturation, improve stability, and prevent activity reduction

Pending Publication Date: 2019-01-11
河北国高生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the enzyme-labeled antibody is placed at 4°C or diluted in a large volume, the immune activity of the enzyme-labeled antibody will decrease rapidly in a short period of time

Method used

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  • Enzyme dilution liquid and preparation method thereof
  • Enzyme dilution liquid and preparation method thereof
  • Enzyme dilution liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] An enzyme diluent consisting of:

[0045] Disodium hydrogen phosphate 2.85g

[0046] Sodium dihydrogen phosphate 0.30g

[0047] 4-Aminoantipyrine 1.00g

[0048] Paracetamol 1.00g

[0049] Sodium chloride 8.00g

[0050] Sodium Caseinate 10.00g

[0051] Carmine 0.02g

[0052] Tween 20 10mL

[0053] Newborn bovine serum 50mL

[0054] Proclin300 1.0mL

[0055] Triton X-100 10mL

[0056] Use purified water for in vitro diagnostic reagents, add to 1000mL.

[0057] Its preparation method comprises the following steps:

[0058] S1. According to the formula of the above-mentioned enzyme diluent, weigh each component for later use;

[0059] S2. Add disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, 4-aminoantipyrine, paracetamol, sodium caseinate, and carmine in sequence to 800 mL of purified water for in vitro diagnostic reagents, and stir at a uniform speed for more than 10 minutes to fully dissolve;

[0060] S3, add Proclin300, newborn bovi...

Embodiment 2

[0063] An enzyme diluent consisting of:

[0064] Disodium hydrogen phosphate 2.5g

[0065] Sodium dihydrogen phosphate 0.25g

[0066] 4-Aminoantipyrine 0.9g

[0067] Paracetamol 0.9g

[0068] Sodium chloride 7.2g

[0069] Sodium Caseinate 9g

[0070] Carmine 0.018g

[0071] Tween 20 9.5mL

[0072] Newborn bovine serum 49mL

[0073] Proclin3000.95mL

[0074] Triton X-100 9.5mL

[0075] Use purified water for in vitro diagnostic reagents, add to 1000mL.

[0076] Its preparation method is with embodiment 1.

Embodiment 3

[0078] An enzyme diluent consisting of:

[0079] Disodium hydrogen phosphate 3g

[0080] Sodium dihydrogen phosphate 0.35g

[0081] 4-Aminoantipyrine 1.1g

[0082] Paracetamol 1.1g

[0083] Sodium chloride 8.8g

[0084] Sodium casein 11g

[0085] Carmine 0.022g

[0086] Tween 20 10.5mL

[0087] Newborn bovine serum 51mL

[0088] Proclin300 1.05mL

[0089] Triton X-100 10.5mL

[0090] Use purified water for in vitro diagnostic reagents, add to 1000mL.

[0091] Its preparation method is with embodiment 1.

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Abstract

The invention belongs to the technical field of in vitro diagnosis reagents, and provides an enzyme dilution liquid, wherein 1000 mL of the enzyme dilution liquid comprise 2.5-3 g of disodium hydrogenphosphate, 0.25-0.35 g of sodium dihydrogen phosphate, 0.9-1.1 g of 4-aminoantipyrine, 0.9-1.1 g of paracetamol, 7.2-8.8 g of sodium chloride, 9-11 g of casein sodium, 0.018-0.022 g of carmine red, 9.5-10.5 mL of Tween 20, 49-51 mL of newborn bovine serum, 0.95-1.05 mL of Proclin300, 9.5-10.5 mL of Triton X-100, and the balance of purified water for in vitro diagnosis reagents. According to the present invention, with the enzyme dilution liquid, the technical problems of poor stability and low immunological activity of the enzyme-labeled antibody in the enzyme-linked immunosorbent assay reagent can be solved.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnostic reagents, and relates to an enzyme diluent and a preparation method thereof. Background technique [0002] Enzyme-linked immunosorbent assay (ELISA) method is widely used in the determination of antibodies and antigens. Its principle is to bind antigens or antibodies to the surface of a certain solid-phase carrier and maintain their immunological activity to form solid-phase antigens or antibodies, that is, immunoadsorption. agent. The antigen or antibody is linked with an enzyme to form an enzyme-labeled antigen or antibody, which not only retains its immunological activity, but also retains the activity of the enzyme. During the determination, the test specimen (determining the antibody or antigen in it) and the enzyme-labeled antibody or antigen react with the antigen or antibody on the surface of the solid-phase carrier in different steps, and the antigen formed on the solid-phase...

Claims

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Application Information

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IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 郭凤学袁大丰
Owner 河北国高生物科技有限公司
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