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Method for detecting ancient wool fabric based on electrochemical luminescence method

A luminescence and electrochemical technology, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis through chemical reactions of materials, etc. It can solve the problems of large interference of impurities, low sensitivity, and unsuitability for cultural relics detection. , to achieve the effects of high sensitivity, improved sensitivity, and good biocompatibility

Active Publication Date: 2019-01-11
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is easily degraded by the influence of light, heat, acid and alkali, microorganisms, etc., resulting in changes in crystallinity, molecular weight and other structures and properties. Therefore, conventional detection methods have low sensitivity and are greatly affected by impurities. Therefore, it is not suitable for the detection of cultural relics. It is necessary to develop a method with good sensitivity and strong specificity for detecting wool fabric cultural relics

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A) Weigh 10mg of cadmium selenide / zinc sulfide quantum dots, 58mg of polymethyl methacrylate, 38mg of polymaleic anhydride-octadecene copolymer, add 0.8ml of chloroform, and 4.5ml of 1.5mg / ml The sodium dodecylbenzenesulfonate aqueous solution was mixed and treated with an ultrasonic homogenizer, and then the chloroform was evaporated. Then the obtained water-soluble quantum dot beads were centrifuged and purified at a centrifugation rate of 8000 rpm for 8 min, and washed twice with deionized water.

[0038]B) Weigh 0.2 mg of quantum dot beads in step A), add 1.8 µg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 1.2 µg of N-hydroxysuccinyl Amine, 2.6ml of PBS 7.4 buffer, react for 2h.

[0039] C) Mix 4ml of 0.2 mg / ml graphene oxide solution with 180µl of 1mM sodium hexachloroplatinate solution, react in an ice-water bath, then add 0.8mg of sodium borohydride to react for 1h under vigorous stirring, then centrifuge , washed twice with deionized water, ...

Embodiment 2

[0055] A) Weigh 10mg of cadmium selenide / zinc sulfide quantum dots, 60mg of polymethyl methacrylate, 40mg of polymaleic anhydride-octadecene copolymer, add in 1ml of chloroform, and mix with 5ml of 1.5mg / ml decane The aqueous solution of sodium dialkylbenzenesulfonate was mixed and treated with an ultrasonic homogenizer, after which the chloroform was evaporated. Then the obtained water-soluble quantum dot beads were centrifuged and purified at a centrifugation rate of 10000 rpm for 10 min, and washed three times with deionized water.

[0056] B) Weigh 0.21 mg of quantum dot beads in step A), add 2 µg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 1.3 µg of N-hydroxysuccinyl Amine, 2.7ml of PBS 7.4 buffer, reacted for 2h.

[0057] C) Mix 4ml of 0.25 mg / ml graphene oxide solution with 200µl of 1mM sodium hexachloroplatinate solution, react in an ice-water bath, then add 1mg of sodium borohydride to react for 1h under vigorous stirring, then centrifuge. Wash 3...

Embodiment 3

[0073] A) Weigh 10mg of cadmium selenide / zinc sulfide quantum dots, 62mg of polymethyl methacrylate, 42mg of polymaleic anhydride-octadecene copolymer, add 1.2ml of chloroform, and 5.5ml of 1.5mg / ml The sodium dodecylbenzenesulfonate aqueous solution was mixed and treated with an ultrasonic homogenizer, and then the chloroform was evaporated. Then, the obtained water-soluble quantum dot beads were centrifuged and purified at a centrifugation rate of 12000 rpm for 12 min, and washed 4 times with deionized water.

[0074] B) Weigh 0.22 mg of quantum dot beads in step A), add 2.2 µg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 1.4 µg of N-hydroxysuccinyl Amine, 2.8ml of PBS 7.4 buffer solution, reacted for 2h.

[0075]C) Mix 4ml of 0.3 mg / ml graphene oxide solution with 220µl of 1mM sodium hexachloroplatinate solution, react in an ice-water bath, then add 1.2mg of sodium borohydride to react for 1h under vigorous stirring, then centrifuge , washed 4 times wit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Abstract

The invention relates to the field of detection of cultural relics and discloses a method for detecting an ancient wool fabric based on an electrochemical luminescence method. The method comprises thefollowing steps: firstly, preparing a cadmium selenide / zinc sulfide quantum dot bead and marking on platinum-modified graphene oxide; then adsorbing a wool keratin antibody to form a probe and incubating a cultural relic modified on a working electrode and wool keratin for a period of time respectively; and then judging whether the cultural relic sample is the wool fabric or not according to a fluorescence signal obtained by electrochemical scanning. The method disclosed by the invention has the characteristics of intuition, accuracy and high sensitivity.

Description

technical field [0001] The invention relates to the field of detection of cultural relics, in particular to a method for detecting ancient woolen fabrics based on an electrochemiluminescence method. Background technique [0002] China has been a big country of textiles since ancient times. The textiles produced are rich in variety, exquisite in craftsmanship, comfortable and breathable. Ancient Chinese wool fabrics are mainly made of wool as raw material. The protein content of wool is about 99%. Wool keratin is composed of 18 kinds of amino acids, of which cystine residues account for 12%. It is easily degraded by the influence of light, heat, acid and alkali, microorganisms, etc., resulting in changes in structure and properties such as crystallinity and molecular weight. Therefore, conventional detection methods have low sensitivity and are greatly affected by impurities. Therefore, it is not suitable for the detection of cultural relics. It is necessary to develop a met...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/327G01N27/30
CPCG01N21/76G01N27/308G01N27/3275
Inventor 王秉李青青欧阳毅刘林帅彭志勤胡智文
Owner ZHEJIANG SCI-TECH UNIV
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