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Mixed staining solution for section used for observation of xylem parenchyma, and preparation method for section

A technique for parenchyma and staining solution, applied in the field of biological slice preparation, can solve the problems of complex preparation method, long time-consuming, difficult to distinguish between parenchyma and fibrous tissue, etc., and achieves the effect of good staining effect.

Inactive Publication Date: 2019-01-11
RES INST OF FORESTRY NEW TECH CHINESE ACAD OF FORESTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a section mixed staining solution for observing xylem parenchyma, which can solve the problems that the existing xylem section preparation method is complicated, takes a long time and is difficult to distinguish parenchyma and fibrous tissue

Method used

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  • Mixed staining solution for section used for observation of xylem parenchyma, and preparation method for section
  • Mixed staining solution for section used for observation of xylem parenchyma, and preparation method for section
  • Mixed staining solution for section used for observation of xylem parenchyma, and preparation method for section

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Embodiment 1

[0043] Embodiment 1: A kind of preparation method of the mixed dyeing solution that is used for xylem slice dyeing

[0044] The preparation steps include: dissolving safranin in ethanol with a concentration of 50% by volume to prepare a safranin ethanol solution with a concentration of 0.010 g / ml; dissolving alcian blue in pure water to prepare an alcian blue with a concentration of 0.010 g / ml Blue aqueous solution; mix safranin ethanol solution and Alcian blue aqueous solution at a volume ratio of 35:65 to prepare a mixed staining solution.

Embodiment 2

[0045] Embodiment 2: A kind of preparation method for the mixed dyeing liquid that is used for xylem slice dyeing

[0046] The preparation steps include: dissolving safranin in ethanol with a concentration of 50% by volume to prepare a safranin ethanol solution with a concentration of 0.010 g / ml; dissolving alcian blue in pure water to prepare an alcian blue with a concentration of 0.010 g / ml Blue aqueous solution; mix safranin ethanol solution and Alcian blue aqueous solution at a volume ratio of 40:60 to prepare a mixed staining solution.

Embodiment 3

[0047] Embodiment 3: A kind of preparation method for the mixed dyeing liquid that is used for xylem slice dyeing

[0048] The preparation steps include: dissolving safranin in ethanol with a concentration of 50% by volume to prepare a safranin ethanol solution with a concentration of 0.010 g / ml; dissolving alcian blue in pure water to prepare an alcian blue with a concentration of 0.010 g / ml Blue aqueous solution; mix safranin ethanol solution and Alcian blue aqueous solution at a volume ratio of 30:70 to prepare a mixed staining solution.

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Abstract

The invention discloses a mixed staining solution for a section used for observation of xylem parenchyma, and a preparation method for the section. The mixed staining solution for the section comprises an ethanol solution of safranine and an aqueous solution of Alcian blue, wherein a volume ratio of the ethanol solution of safranine to the aqueous solution of Alcian blue is (30-40): (60-70); and the concentrations of the ethanol solution of safranine and the aqueous solution of Alcian blue are both 0.008 to 0.012 g / ml. In the mixed staining solution for the section, saffron dyes fibrous tissueand Alcian blue dyes parenchymatous tissue; so the parenchymatous tissue, fibrous tissue and ducts (or tracheid) of the stained section can be clearly observed at the same time, and the fibrous tissue and parenchymatous tissue of xylem can be clearly distinguished. Compared with staining solutions consisting of the ethanol solution of safranine and the aqueous solution of Alcian blue in other ratios, the mixed staining solution of the invention has better dyeing effect and shorter section preparation time; and the mixed staining solution is applicable to all the woody plants and overcomes thedisadvantage that staining agents used in conventional section preparation methods are hard to distinguish the fibrous tissue and the parenchymatous tissue by color.

Description

technical field [0001] The invention relates to the technical field of preparation of biological slices, in particular to a method for preparing slices for observing xylem parenchyma mixed with staining solution and slices. Background technique [0002] Making xylem slices is a common method for studying plant anatomy. The xylem of woody plants is mainly composed of vessels (or tracheids), fibrous tissue and parenchyma. Parenchyma is the living cell in xylem, which has the function of storing and transferring water and nutrients. In order to strengthen the research on the functional and strategic differences among plant species, it is urgent to study the characteristics of parenchyma in woody plants. [0003] However, the currently commonly used stains for making xylem slices are all solid colors for parenchyma and fibrous tissue, or the color distinction between the two is not obvious, so it is difficult to distinguish parenchyma and fibrous tissue by color. For example, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/286G01N1/30G01N2001/302
Inventor 陈志成李姗万贤崇
Owner RES INST OF FORESTRY NEW TECH CHINESE ACAD OF FORESTRY
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