A FISH detection probe and kit for aortic dissection

Abstract

A FISH detection probe and kit for aortic dissection are provided. The probe comprises a FISH fluorescent probe used for detecting MiR-4769-3P, has-MiR-636, has-MiR-142-3p, has-MiR-425-3p and has-MiR-191-3p FISH fluorescent probe, the nucleotide sequence of which is as follows: SEQ ID NO: 1-5. The kit of the invention can be used for molecular diagnosis of large artery dissection, auxiliary etiological diagnosis of large artery dissection, and differentiation of dissecting and non-dissecting tissues for surgical and pathological judgment of lesion range.

Description

Technical field [0001] The present invention relates to biological detection technology, in particular to a probe and kit for FISH detection of aortic dissection. Background technique [0002] Aortic dissection (AD) refers to the rupture of the aortic intima caused by various reasons. The blood enters the aortic wall through the intima tear and forms a false cavity between the intima and the adventitia. This is a severely life-threatening aortic disease. Once it ruptures, the fatality rate is extremely high or the acute ischemia of important organs (such as: heart, brain, kidney, intestine, etc.) causes severe complications, and the condition is dangerous. In recent years, in my country and the world, the incidence and detection rate of AD are increasing year by year. Early diagnosis and reasonable treatment are the key to saving patients' lives. AD is a complex disease, and the etiology and pathogenesis are still unknown. Related high-risk factors include hypertension, smoking...

AI Technical Summary

Problems solved by technology

[0008] Based on the above, although a large number of theoretical studies on the possibility of early diagnosis of aortic dissection have been disclosed in the prior art, there is still no detection kit that can effectively perform early diagnosis of aortic dissection.

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