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A composite microbial agent and a preparation method and application thereof

A technology of compound microbial inoculants and inoculants, applied in the field of microorganisms, can solve the problems of inhibited growth of strains, reduced biomass, and insignificant effects of strains, and achieve the effects of reducing impact, reducing availability, and solving soil heavy metal pollution.

Inactive Publication Date: 2019-01-11
泽明环境发展有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The effect of using heavy metal-resistant strains alone is not obvious, the growth of the strains is inhibited in the later stage, and the biomass is reduced, while the combined use of multiple strains with high tolerance to heavy metals is often accompanied by growth competition between different strains, so it provides a product with significant mercury resistance and It is of great significance and broad prospects for the remediation of soil contaminated with heavy metal mercury by the composite microbial agent with mutual promotion of growth

Method used

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  • A composite microbial agent and a preparation method and application thereof
  • A composite microbial agent and a preparation method and application thereof
  • A composite microbial agent and a preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0062] A compound microbial bacterial agent, comprising Saccharomyces cerevisiae, Fusarium oxysporum, Alternaria tenosporum and Pseudomonas putida, its preparation method is as follows:

[0063] (1) Activation of strains: respectively activate Saccharomyces cerevisiae, Fusarium oxysporum, Alternaria tenosporum and Pseudomonas putida respectively, activate 3 generations, expand cultivation to secondary seed liquid, inoculate into seed tanks for further expansion cultivation , to obtain the seed liquid for fermentation;

[0064] (2) Enlarged cultivation of fermenter: inoculate the seed liquid obtained in step (1) into the fermenter respectively, the inoculation ratio is that the seed liquid for fermentation accounts for 5% of the fermented liquid, aeration and agitation culture, ventilation volume 2.8m 3 / h, the stirring speed is 120rpm, ferment for 5 days, the fermentation temperature is 25°C, and the bacterial suspension is obtained;

[0065] (3) The bacterial suspension obta...

Embodiment 2

[0068] A compound microbial bacterial agent, comprising Saccharomyces cerevisiae, Fusarium oxysporum, Alternaria tenosporum and Pseudomonas putida, its preparation method is as follows:

[0069] (1) Strain activation: respectively activate Saccharomyces cerevisiae, Fusarium oxysporum, Alternaria adenocarcinum and Pseudomonas putida, activate 4 generations, expand culture to secondary seed liquid, inoculate into seed tanks for further expansion culture , to obtain the seed liquid for fermentation;

[0070] (2) Enlarged cultivation of fermenter: inoculate the seed liquid obtained in step (1) into the fermenter respectively, the inoculation ratio is that the seed liquid for fermentation accounts for 4% of the fermented liquid, aeration and agitation culture, ventilation volume 2.1m 3 / h, the stirring speed is 150rpm, ferment for 6 days, the fermentation temperature is 25°C, and the bacterial suspension is obtained;

[0071] (3) The bacterial suspension obtained in step (2) is ad...

Embodiment 3

[0074] A compound microbial bacterial agent, comprising Saccharomyces cerevisiae, Fusarium oxysporum, Alternaria tenosporum and Pseudomonas putida, its preparation method is as follows:

[0075] (1) Strain activation: respectively activate Saccharomyces cerevisiae, Fusarium oxysporum, Alternaria tenosporum and Pseudomonas putida, activate 5 generations, expand culture to secondary seed liquid, inoculate into seed tanks for further expansion culture , to obtain the seed liquid for fermentation;

[0076] (2) Enlarged cultivation of fermenter: inoculate the seed liquid obtained in step (1) into the fermenter respectively, the inoculation ratio is that the seed liquid for fermentation accounts for 2% of the fermented liquid, aeration and agitation culture, ventilation rate 1m 3 / h, the stirring speed is 100rpm, ferment for 3 days, the fermentation temperature is 27°C, and the bacterial suspension is obtained;

[0077] (3) The bacterial suspension obtained in step (2) is added in ...

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Abstract

The invention provides a composite microbial agent and a preparation method and application thereof, wherein the composite microbial agent comprises Saccharomyces cerevisiae, Fusarium oxysporum, Alternaria tenuiflora and Pseudomonas putida. The invention combines Saccharomyces cerevisiae, Fusarium oxysporum, Alternaria tenuiflora and Pseudomonas putida to synergize the four strains, regulate the metabolic balance among the bacteria, make the compound bacterium highly effective and stable to pollute the mercury in the soil, and reduce the mercury content in the plant.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a composite microbial bacterial agent and its preparation method and application, in particular to a composite microbial bacterial agent for treating farmland polluted by heavy metal mercury and its preparation method and application. Background technique [0002] Mercury is one of the pollutants of wide global concern. The soil in many places at home and abroad has been polluted by mercury to varying degrees. Mercury pollution in my country is mainly concentrated in Guigui, Jilin, Shanxi, Hubei, Liaoning and Chongqing. The sources of mercury in soil are very extensive, including soil parent material, dry and wet deposition of atmospheric mercury, discharge of mercury-containing wastewater, accumulation of mercury-containing solid waste, etc. Mercury is extremely harmful, not only causing amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease and many other d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14B09C1/10C12R1/865C12R1/77C12R1/40C12R1/645
CPCB09C1/105C12N1/14C12N1/18C12N1/20
Inventor 刘春燕张梅华阮巍吴轩王亚超
Owner 泽明环境发展有限公司
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