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Method for differentiating black tea with different producing areas

A technology of black tea, origin, applied in the direction of material separation, analysis of materials, measuring devices, etc.

Inactive Publication Date: 2019-01-08
云南清莲生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the method of distinguishing black tea from different origins by using the aroma composition information of tea leaves combined with chemometric methods (CA and PCA)

Method used

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  • Method for differentiating black tea with different producing areas
  • Method for differentiating black tea with different producing areas
  • Method for differentiating black tea with different producing areas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Using automatic headspace solid-phase microextraction combined with gas chromatography-mass spectrometry and chemometric methods to distinguish black tea from different origins (Yunnan Dian black tea and Hubei Ning black tea)

[0044] (1) Material preparation: 9 pieces of Dianhong tea are from Yunnan Province, numbered YB1-YB9, all grades are first grade, produced in 2013, and 3 pieces of Ninghong tea are from Hubei Province, numbered HB1-HB3.

[0045] (2) Sample preparation: The PDMS / DVB / CAR extraction fiber head was aged at 250°C for 60 min at the GC inlet. Accurately weigh 2g of tea powder for each tea sample, put it into a 20mL headspace bottle, add 5mL of boiling distilled water for brewing, immediately seal the bottle mouth, and brew for 2 minutes. Then place it in a headspace incubator with a rotation speed of 250r / min. After equilibrating at 80°C for 10 minutes, the autosampler inserts the extraction head into the headspace of the headspace bottle to ...

Embodiment 2

[0048] Example 2: Using automatic headspace solid-phase microextraction combined with gas chromatography-mass spectrometry and chemometric methods to distinguish Yunnan Dian black tea from black tea from three other provinces

[0049] (1) Material preparation: 13 Yunnan black tea samples were from Yunnan Province, numbered YB1-YB13, and the other 9 black tea samples were from Anhui, Hubei, and Fujian Provinces, numbered Z1-Z9. All samples are Grade 1 and produced in 2013.

[0050] (2) Sample preparation: The PDMS / DVB / CAR extraction fiber head was aged at 250°C for 60 min at the GC inlet. Accurately weigh 2g of tea powder for each tea sample, put it into a 20mL headspace bottle, add 5mL of boiling distilled water for brewing, immediately seal the bottle mouth, and brew for 2 minutes. Then place it in a headspace incubator with a rotation speed of 250r / min. After equilibrating at 80°C for 10 minutes, the autosampler inserts the extraction head into the headspace of the headspac...

Embodiment 3

[0053] Example 3: Using automatic headspace solid-phase microextraction combined with gas chromatography-mass spectrometry and chemometric methods to distinguish between black tea produced in Fujian and black tea produced in Hubei

[0054] (1) Material preparation: 2 samples of Fujian black tea, numbered FB1-FB2, 6 black tea samples from Hubei, numbered HB1-HB6, all teas were of the same grade and year of production.

[0055] (2) Sample preparation: The PDMS / DVB / CAR extraction fiber head was aged at 250°C for 60 min at the GC inlet. Accurately weigh 2g of tea powder for each tea sample, put it into a 20mL headspace bottle, add 5mL of boiling distilled water for brewing, immediately seal the bottle mouth, and brew for 2 minutes. Then place it in a headspace incubator with a rotation speed of 250r / min. After equilibrating at 80°C for 10 minutes, the autosampler inserts the extraction head into the headspace of the headspace bottle to extract for 60 minutes. min, and start the i...

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Abstract

The invention discloses a method for differentiating black tea with different producing areas. Fragrance components in tea are extracted by a fully-automatic headspace solid-phase microextraction (HS-SPME) method, the fragrance components of the tea are separated and identified by combining gas chromatography-mass spectrometry (GC-MS), and a clustering and main component model is constructed by utilizing the relative content of the identified fragrance components of the tea and combining a chemometrics method (CA and PCA), so that the black tea in different producing areas can be differentiated. The invention provides a new effective evaluation and control method for examining and identifying the quality of the tea (particularly producing area judgment based on the fragrance components). The method is simple and practical to operate, low in cost and environmentally friendly; and an efficient, objective and normalized method can be provided for performing quality evaluation and controlon the tea.

Description

technical field [0001] The invention belongs to the technical field of tea, and in particular relates to a method for distinguishing black tea from different origins by using full-automatic headspace solid-phase microextraction combined with gas chromatography mass spectrometry and chemometric methods. Background technique [0002] Black tea is one of the most popular teas in the world, accounting for 78% of the total global tea production. Black tea is currently the tea with the widest consumption area, the largest production volume and the largest total international trade volume in the world. Black tea originated in China. With the integration of Eastern and Western cultures and the change of people's life rhythm, the consumption area and drinking population of black tea have greatly increased, and the black tea market is gradually expanding. [0003] Black tea is a fully fermented tea, mainly produced in Fujian, Hubei, Anhui, and Yunnan. It is mainly divided into Soucho...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 孟庆雄
Owner 云南清莲生物科技有限公司
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