Vibrio marinopraesens as well as separation method and application thereof

Abstract

The invention discloses vibrio marinopraesens and a separation method thereof. Vibrio marinopraesens HN897 generating agarase is separated and obtained from seawater samples of coastal areas in SouthChina, and is preserved in China General Microbiological Culture Collection Center on August 8, 2018 with the preservation number of CGMCC NO.16232. Through species identification, the vibrio marinopraesens HN897 is shown as Vibrio astriarenae. The bacterial strain cultured on a solid agar plate shows that the bacterial strain has the capacity for degrading agar, and the activity of agarase secreted from 2 culturing liquid (TSB and LB) shows that the bacterial strain has high-yield capacity of agarase. Therefore, the vibrio marinopraesens HN897 has wide application and market prospects in thebiopharmaceutical fields of agaro-oligosaccharides and the like, and besides, establishes a basis for subsequent preparation of enzyme products being high in vitality and high in purity.

Description

technical field [0001] The invention relates to a strain of marine vibrio, in particular to a strain of marine vibrio and its isolation method and application, belonging to the technical field of biomedicine. Background technique [0002] Agarase is a class 1 glycoside hydrolase that can catalyze the decomposition of agarose into oligosaccharides. Agarose is a linear macromolecule formed by alternating linkages of D-galactose and 3,6-endo-α-L-galactose through α-1,3 and β-1,4 glycosidic bonds. [0003] According to the difference of the glycosidic bond catalyzed, agarase can be divided into two types: α-agarase (EC3.2.1.158) and β-agarase (EC3.2.1.81); the former specifically acts on The α-1,3 glucosidic bond of agarose produces agarose oligosaccharides with β-D-galactose as the non-reducing end and 3,6-endether-α-L-galactose as the reducing end Agaro-oligosaccharides (Agaro-oligosaccharides), while the latter specifically acts on the β-1,4 glycosidic bond of agarose to ge...

AI Technical Summary

Problems solved by technology

[0007] At present, the research method of agarase has been relatively mature, and foreign research started earlier, and a variety of bacteria capable of producing agarase and corresponding agarases have been found, such as AgaA, AgaB and AgaC, etc. The focus of research has also been to extract agarase from bacteria Turn to the research on the agarase gene and obtain more and purer agarase by recombining the gene in E. coli for prokaryotic expression; domestic research is relatively late, but on the basis of foreign research, some breakthroughs have also been made

[0008] Summarizing the current research at home and abroad, there are the following problems to be solved, one part is that there are few natural sources of α-agarase, so there are fewer studies on α-agarase; the other part is that the high-purity agarase obtained by current research is equivalent to Expensive, the by-products generated by the degradation of agar by agarase have not been widely used in industrial production

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