MRFFT1 cell

Abstract

The invention relates to an MRFFT1 cell and a preparation method of the MRFFT1 cell, a mutation polypeptide is screened through the predication of an epitope, the sequence of an expression gene of themutation polypeptide is connected and synthesized; meanwhile, an MVA virus vector is constructed, an MVA virus is packaged, an APC (Antigen Presenting Cell) is transfected, the transformation of a specific MV cell is finished, the APC is cultured in vitro together with a PBMC (Peripheral Blood Mononuclear Cell) separated from the peripheral blood, the effective polypeptide is screened, a common Tcell is transformed into an RFF cell having more accurate lethality through the second impact stimulated by the accurate effective polypeptide; the transformation is conducted through the TCR-T technical principle; an immunosuppressive target of the transformed T cell is knocked off through the gene editing technology, the specific lethal T cell is protected accurately against in-vivo suppression, and the lethality of the T cell on a tumor cell is improved.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a MRFFT1 cell and a preparation method thereof. Background technique [0002] At present, in terms of tumor specific immunotherapy, the existing LAK, DC, CIK, DC-CIK cells and methods have basically proved to be ineffective, while cell technologies such as NK, CAR-NK, and TIL have yet to mature. CAR- T cells also have defects in safety and solid tumor treatment. The prior art generally transforms DC cells, and DC presents T cells to produce specific killing. Some laboratories are trying to use viruses as vectors to transfect and present T cells to induce specific killing of T cells. We have also used mutant mixed peptides to directly stimulate PBMC and induce T cells. There are also laboratories using TCR-T technology to target and present MAGE A3 antigen. [0003] The above treatment methods are immature, especially the in vitro induction of DC cells and DC cells to load tum...

AI Technical Summary

Problems solved by technology

[0004] None of the above schemes considers the self-protection technology of T cells, so that a small number of specific T cells directly face the powerful tumor immune microenvironment

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