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Bioreactor

A bioreactor, the other side of the technology, applied in the field of medical devices, can solve the problems of limited material exchange capacity, poor cell stability, uneven perfusion, etc., to achieve convective exchange, flexible installation, and low shear force Effect

Pending Publication Date: 2019-01-04
HEXAELL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(3) Perfusion bed or scaffold bioreactor: This bioreactor is to plant hepatocytes on a perfusion bed or scaffold, which has the advantage of direct contact with cells, which increases the transport of substances and promotes the formation of three-dimensional structures. It is also easy to expand the cell capacity; its disadvantage is uneven perfusion and easy clogging
Its advantage is that all cells have the same microenvironment, there is a large amount of space for cell culture, and the occurrence of immune reactions is reduced; the disadvantage is poor cell stability and limited material exchange capacity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Design of the bioreactor of the present invention

[0049] (1) Analysis of inlet position, laminate spacing and flow field

[0050] The through holes at the center of the top and bottom cell culture plates in the bioreactor were sealed to prevent the liquid from flowing directly, and the cell culture plates were set at two intervals of 0.5mm and 2mm respectively to investigate the flow of the liquid. Each structure is as Figure 1 to Figure 4 shown.

[0051] (1) The through-hole sealing of the bottom culture plate VS the through-hole sealing of the top culture plate

[0052] From the perspective of liquid flow, after the through hole of the bottom cell culture plate is closed, the liquid flowing in from the upper inlet will be forced to flow to both ends at the bottom layer, and then continue to flow vertically upward through the small hole on the bottom cell culture plate. In this case, each small hole on the bottommost cell culture plate is approximatel...

Embodiment 2

[0062] Example 2 Bioreactor of the present invention

[0063] Figure 11 to Figure 15 Shown, a kind of bioreactor comprises housing 1, top cover 2, and a plurality of cell culture plates 6, and housing 1 has open upper end and closed lower end, wherein the lower end is provided with housing 1 internal fluid communication Inlet 3, the top cover 2 covers the open upper end of the housing 1 and is provided with an outlet 4 in fluid communication with the inside of the housing 1, a plurality of cell culture plates 6 are spaced from each other and arranged in parallel inside the housing 1, each cell culture The plate 6 is provided with a through hole 51 at the center and a plurality of small holes 61 distributed around the through hole 51 , and the through holes 51 of a plurality of cell culture plates are aligned with each other to form the channel 5 .

[0064] The through hole 51 of the top cell culture plate 62 closest to the top cover 2 is closed, thereby sealing the upper e...

Embodiment 3

[0073] Example 3 Use of the bioreactor of the invention

[0074] The bioreactor of Example 2 was used.

[0075] Open the outlet 4 on the top cover 2, and input the liver cell suspension from the inlet 3 at the lower end of the housing 1. After the suspension is basically filled with the entire bioreactor, close the inlet 3 and let it stand for 3-4 hours to make the cells in the cell Adherent growth occurs on the surface of the culture plate.

[0076] Connect the bioreactor to the human body through other components of the biological blood purification system, and start the circulation of the plasma to be purified.

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Abstract

The invention provides a bioreactor. The bioreactor comprises a housing, a top cover and a plurality of cell culture plates, the housing is provided with an opened upper end and a closed lower end, and an inlet communicated with fluid inside the housing is formed in the lower end; the top cover covers the opened upper end of the housing, and an outlet communicated with fluid inside the housing isformed; the plurality of cell culture plates are spaced apart from each other and disposed in parallel within the housing, through holes and a plurality of small holes distributed around the through holes are formed in the centers of each cell culture plate, and the through holes of the plurality of cell culture plates are aligned with each other to form a center channel. The bioreactor is more advantageous for plasma to be purified diffusing around the cell culture plates and exchanging sufficient substances with cells.

Description

technical field [0001] The invention relates to a medical device, especially a bioreactor. Background technique [0002] The biological blood purification system is the closest artificial liver support system to the normal liver. By combining hepatic cell culture technology with blood purification technology, it can replace the functions of liver detoxification, biosynthesis, secretion and metabolism in a relatively comprehensive way. The most innovative and challenging treatment for patients with liver failure. [0003] The basic principle of the biological blood purification system is to place the hepatocytes cultured and proliferated in vitro in a special bioreactor, and through material exchange and biological interaction with the hepatocytes, the hepatocytes are used to secrete endogenous active substances and transform exogenous toxins To play the role of biological blood purification, the endogenous active substances include various proteins, metabolic enzymes and ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/04A61M1/16
CPCA61M1/1623C12M23/12C12M23/38
Inventor 武之涛杨晨曦
Owner HEXAELL BIOTECH
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