Somatic whole-cell vaccine composition for treating chronic diseases and preparation method thereof

A whole-cell vaccine, a technology for chronic diseases, applied in the field of autologous whole-cell vaccine formulations for the treatment of chronic diseases and the field of preparation thereof, can solve problems such as no obvious breakthrough, and achieve the effects of preventing disease recurrence, improving curative effects, and improving quality of life

Active Publication Date: 2019-01-04
JIANGSU SUPERBIO LIFE SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no obvious breakthrough in the preparation of autologous whole cell vaccines for the treatment of chronic diseases. Therefo

Method used

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  • Somatic whole-cell vaccine composition for treating chronic diseases and preparation method thereof
  • Somatic whole-cell vaccine composition for treating chronic diseases and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for preparing an autologous whole cell vaccine for treating chronic diseases, comprising the following steps:

[0031] Step 1: Collect, collect about 50ml of peripheral blood from the human body with chronic diseases; centrifuge the collected blood in a centrifuge at a speed of 1500rpm for 8 minutes, suck out the upper serum and transfer it to another centrifuge tube In the process, the plasma was placed in a 56°C water bath for 30 minutes of inactivation, and then centrifuged at a speed of 1000 rpm for 8 minutes. The processed supernatant was transferred to another centrifuge tube and placed. The ambient temperature is 4°C;

[0032] Step 2: Separation, separating peripheral blood and extracting autologous cells; adding the obtained plasma whole blood to normal saline to dilute 2 times, and slowly adding the above precipitated or diluted blood to the lymphocyte separation solution, and the lymphocyte separation solution according to 1: The ratio of 1 was added...

Embodiment 2

[0041] A method for preparing an autologous whole cell vaccine for treating chronic diseases, comprising the following steps:

[0042] Step 1: Collection, about 120ml of peripheral blood is collected from the human body with chronic diseases; the collected blood is centrifuged in a centrifuge at a speed of 3000rpm, and the centrifugation time is 8 minutes. The upper serum is sucked out and transferred to another centrifuge tube In the process, the plasma was placed in a 56°C water bath for 30 minutes of inactivation, and then centrifuged at a speed of 2800 rpm for 8 minutes. The treated supernatant was transferred to another centrifuge tube and placed The ambient temperature is 4°C;

[0043] Step 2: Separation, separating peripheral blood and extracting autologous cells; adding the obtained plasma whole blood to normal saline to dilute 2 times, and slowly adding the above precipitated or diluted blood to the lymphocyte separation solution, and the lymphocyte separation solutio...

Embodiment 3

[0052] A preparation method of autologous whole cell vaccine for treating chronic diseases, comprising the following steps:

[0053] Step 1: Collection, collect about 100ml of peripheral blood from people with chronic diseases; centrifuge the collected blood in a centrifuge, the speed of the centrifuge is 2300rpm, the centrifugation time is 8 minutes, the upper serum is sucked out and transferred to another centrifuge tube , the plasma was placed in a 56°C water bath for 30 minutes of inactivation, and then centrifuged at 1800 rpm for 8 minutes, and the treated supernatant was transferred to another centrifuge tube and placed. The ambient temperature is 4°C;

[0054] Step 2: separate, separate peripheral blood and extract autologous cells; add the gained plasma whole blood to normal saline to dilute 2 times, and slowly add the above precipitation or diluted blood dilution to contain the lymphocyte separation solution, and the lymphocyte separation solution is according to 1: 1...

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PUM

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Abstract

The invention discloses a somatic whole-cell vaccine composition for treating chronic diseases. The composition includes somatic cells, monoclonal antibodies, cell factors, a cell cracking agent and normal saline. The invention also discloses a preparation method of a somatic whole-cell vaccine for treating the chronic diseases, and the method includes the steps of collecting, separation, extraction, culturing, amplification, collecting, splitting decomposition, purification and agent preparation. The vaccine is prepared by utilizing variational cells in blood of the human body and can stimulate the human body to carry out a specific immunity reaction on variational antigens, correspondingly immune cells and the monoclonal antibodies both of which have a specific lethal effect are generated and circularly identify and kill the variational cells carrying the variational antigens in the human body, metabolic waste generated by the variational cells are removed, and the purposes of preventing relapse of the diseases and improving the living quality are achieved. At present, it is proved by experiments that the vaccine has a great therapeutic effect on patients suffering from diabetesand skin diseases.

Description

technical field [0001] The invention relates to the technical field of immune cell therapy, in particular to an autologous whole cell vaccine formula for treating chronic diseases and a preparation method thereof. Background technique [0002] Whole-cell vaccines can safely and effectively activate the immune system and stimulate the body to produce natural killer cells. It takes about a week for the immune response to reach the mutated cells to produce the primary response of specific monoclonal antibodies, and it takes about two weeks to reach the secondary response of mutated cell-specific monoclonal antibodies, a variety of cells involved in the innate immune response Factors play a regulatory role in various immune responses, so that vaccines can make full use of the immune system to completely kill heterogeneous and variable mutant cell populations. [0003] At present, there is no obvious breakthrough in the preparation of autologous whole cell vaccines for the treat...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61P3/10A61P17/00C12N5/078
CPCA61K39/0008C12N5/0634C12N2501/599C12N2501/515C12N2501/2315C12N2501/2302C12N2509/00
Inventor 赵刚
Owner JIANGSU SUPERBIO LIFE SCI CO LTD
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