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Method for rebuilding marrow microenvironment

A technology of microenvironment and bone marrow, which is applied in the direction of medical formula, medical preparations containing active ingredients, drug delivery, etc., can solve the problems of pathological bone marrow microenvironment remodeling, restore normal bone marrow hematopoietic function, delay pathological process, prolong lifetime effect

Active Publication Date: 2018-12-21
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, there is no effective approach to address the remodeling of the pathological bone marrow microenvironment

Method used

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  • Method for rebuilding marrow microenvironment
  • Method for rebuilding marrow microenvironment
  • Method for rebuilding marrow microenvironment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Establishment of a mouse leukemia (CMML / JMML-like) model of progressive destruction of the bone marrow microenvironment

[0063] Leukocytes (stromal cell-depleted) from NrasG12D mutant mice (LSL NrasG12D / +; Vav-Cre) or control mice (CD45.2) were transplanted into sublethally irradiated (6.5Gy) CD45 .1 Recipient mice to construct a mouse leukemia model of chronic myelomonocytic leukemia (CMML / JMML-like). The burden and proliferation of tumor cells in peripheral blood were analyzed monthly by flow cytometry. In the leukemia mouse model induced by NrasG12D, the different stages of leukemia development were defined according to the proportion of myeloid cells in the peripheral blood of the mice. When the myeloid cells (Mac1) in the peripheral blood of the mice were greater than 20% and less than 60%, It is the pre-leukemic phase (Pre-leukemic phase, sometimes referred to as PL phase hereinafter), and when Mac1>60%, it is the leukemia phase (Leukemic phase).

Embodiment 2

[0064] Example 2 Histological analysis proves that endosteal osteoblasts are gradually exhausted in the bone marrow microenvironment during the incubation period of leukemia

[0065] In order to investigate whether there is a gradual depletion of the bone marrow mesenchymal microenvironment during the incubation period of NrasG12D-induced leukemia, we first sacrificed control mice and NrasG12D recipient mice in the incubation period of leukemia and isolated the tibia and femur, and treated them with 4% paraformaldehyde. Hematoxylin-eosin staining and immunohistochemical staining for osteocalcin were performed after fixation. Hematoxylin-eosin staining ( figure 1 A) The osteoblasts (osteoblast, sometimes referred to as OB) near the endosteum in the NrasG12D recipient mice showing the leukemia incubation stage have been exhausted; osteocalcin histochemical staining ( figure 1 B) Osteoblasts near the endosteum have been depleted in NrasG12D recipient mice showing the leukemia la...

Embodiment 3

[0066] Example 3 Cytological analysis proves that with the development of leukemia, the mesenchymal stromal cells (MSCs) in the bone marrow microenvironment are gradually exhausted

[0067] To assess the depletion of functional mesenchymal stromal cells in NrasG12D recipient mice, we performed a fibroblast colony-forming unit assay (CFU-F). After first sacrificing the mice, the bone marrow nucleated cells (BMNC) were aseptically isolated and counted, and 1x10 cells from each mouse in the three groups 6 BMNC with 2mL MesenCult TM Complete medium (Catalog 05512, StemCell Technology) was suspended and seeded in a single well of a six-well plate. In a cell culture incubator at 37°C, 5% CO 2 Cultivate under the same conditions until the 7th day, and continue to culture for 3-7 days after replacing half of the medium on the 7th day. When the culture reaches the 10th-14th day, fix the cells with pre-cooled ethanol and perform Wright's Giemsa staining, and count the formed Number o...

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Abstract

The invention provides a method for rebuilding a marrow microenvironment in a testee whose marrow microenvironment is damaged. The method comprises the step of implanting a composition containing separated mesenchymal stromal cells (MSC) into a marrow cavity of the testee. The method achieves rebuilding the marrow microenvironment, recovers the normal marrow hematopoiesis function, inhibits / delaysthe leukemia pathology progress and obviously prolongs the survival period. Besides, the method can be used for treating blood tumors like leukemia and Aplastic anemia (AA) and has the advantages ofbeing safe, effective and free of side effects.

Description

technical field [0001] The present invention relates to the field of bone marrow microenvironment remodeling, in particular to a method for remodeling bone marrow microenvironment and its application in treating cancer, especially leukemia. Background technique [0002] Chronic myelomonocytic leukemia (CMML) and juvenile myelomonocytic leukemia (JMML) are clonal hematologic malignancies with features of myelodysplasia (MDS) and myeloproliferative disorder (MPN). CMML and JMML are associated with oncogenic point mutations in genes involved in RAS / MAPK signaling, and 25% to 40% of CMML / JMML patients carry somatic mutations in NRAS. It has been reported that mice carrying endogenous mutations of the Nras allele develop a CMML-like disease with a long latency period. There are also reports showing a frequent association between MDS / MPN disease and bone marrow (BM) microenvironment dysfunction in human patients with CMML / JMML as well as in animal models. Aplastic anemia (AA) is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P35/02A61P7/06
CPCA61K9/0019A61K35/28A61P7/06A61P35/02
Inventor 王金勇夏成祥董勇王童洁刘晓飞杜鹃耿阳刘丽娟吴红玲
Owner GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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