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A Genetic Marker Using lrp10 Gene as Eye Muscle Area and Backfat Thickness in Dahe Black Pig

A genetic marker, pig eye muscle technology, applied in the field of genetic markers

Active Publication Date: 2021-09-10
云南东恒经贸集团猪育种有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the market about using the mutation of the pig LRP10 gene sequence as a genetic marker of Dahe black pigs to carry out marker-assisted breeding for eye muscle area and backfat thickness of Dahe black pigs

Method used

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  • A Genetic Marker Using lrp10 Gene as Eye Muscle Area and Backfat Thickness in Dahe Black Pig
  • A Genetic Marker Using lrp10 Gene as Eye Muscle Area and Backfat Thickness in Dahe Black Pig
  • A Genetic Marker Using lrp10 Gene as Eye Muscle Area and Backfat Thickness in Dahe Black Pig

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Embodiment Construction

[0020] see Figure 1-Figure 2 As shown, the technical scheme adopted in this specific embodiment is: its genetic marker nucleotide sequence is as follows:

[0021] ctacttccctccttgttcNtcagtctgaggggacatgatgggctttccattgatgacaggtagctgcgttaaagttaggtgtccctcaggcagggagagggctcacacagagccccttctgcacatggccagagaccacaggccaccaccaccaccatttgggtggctgattttaaaaggtaaagttcttaaagggtcataggcctggacactccatccttgccaaaccgccacccaaaagtggccttaagcacaatgccaattggctgggacttccagctcccaaggggaggatttgggcagggcctgaggttttgcctacagggatccctcccagaaggcctggctcataaaaagagcacaacaaatgcttttgtcccatagctaggtcattgc;所述的核苷酸序列中的N是c或a,导致多重PCR多态性。

[0022] The specific PCR primers of the genetic markers include primer 1, primer 2 and primer 3, the primer 1 is 5'-CTACTTCCCCTCCTTGTTC-3', the primer 2 is 5'-AGTGGCCTTAAGCACTAG-3', the Primer 3 was 5'-GCAATGACCTAGCTATGG-3'.

[0023] A detection method using the LRP10 gene as a genetic marker of eye muscle area and backfat thickness of Dahe black pigs, comprising the following steps:

[0024] Ste...

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Abstract

The invention discloses a genetic marker using LRP10 gene as eye muscle area and backfat thickness of Dahe black pig, which relates to the technical field of pig genetic marker preparation. Its genetic marker nucleotide sequence is as follows: ctacttccctccttgttcNtcag...tagctaggtcattgc; N in the nucleotide sequence is c or a, resulting in multiple PCR polymorphism. The beneficial effects of the present invention are: it can be used to develop diagnostic methods or kits, so that these methods or kits can be used in breeding programs to select pigs carrying favorable alleles, thereby achieving a better selection effect; The Dahe black pig individual containing the AA allele in the LRP10 gene locus can be used as a breeding stock, which can increase the eye muscle area of ​​the offspring of the Dahe black pig and reduce the average backfat thickness.

Description

technical field [0001] The present invention relates to the technical field of preparation of pig genetic markers, in particular to a genetic marker for the eye muscle area and backfat thickness of Dahe black pigs using LRP10 (Low-density lipoprotein receptor-related protein 10) gene mark. Background technique [0002] Lean meat percentage is one of the important economic traits of pigs. Eye muscle area and backfat thickness are important indicators of pig lean meat percentage. Eye muscle area is positively correlated with lean meat percentage, and backfat thickness is negatively correlated with lean meat percentage. . Pig eye muscle area or living body backfat thickness are usually selected to speed up the breeding of pigs and improve the lean meat rate of pigs. With the rise and rapid development of molecular biology techniques, a method combining conventional breeding and molecular marker-assisted selection (MAS) has been developed, which can effectively accelerate the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143
Inventor 李祥刘永刚牛元力
Owner 云南东恒经贸集团猪育种有限公司
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