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Gel for detecting content of organophosphorus and carbamate pesticide and application thereof

A carbamate and organophosphorus technology is applied in measurement devices, preparation of test samples, color/spectral property measurement, etc. Guaranteed effect of enzymatic activity

Inactive Publication Date: 2018-12-18
四川众益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection method is complex to operate and takes a long time period (at least 30min)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The gel used for the content of organophosphorus and carbamate pesticides provided by this embodiment includes the following components and parts by volume: 100 parts of polyvinyl alcohol, 100 parts of chitosan acetic acid solution, and 2 parts of formaldehyde with a mass concentration of 37%. 10 parts of cholinesterase solution with enzyme activity of 0.2U / ml.

[0030] The preparation method of the gel for the content of organophosphorus and carbamate pesticides comprises the steps:

[0031] (1) raw material selection and preparation: choosing polyvinyl alcohol solution, chitosan acetic acid solution, mass concentration is 37% formaldehyde solution and enzyme activity is the cholinesterase solution of 0.2U / ml, described polyvinyl alcohol solution is Prepared by dissolving 1 part by mass of polyvinyl alcohol 1788 in 50 parts by mass of deionized water, the chitosan acetic acid solution is prepared by completely dissolving 1 part by mass of chitosan in 50 parts by volume...

Embodiment 2

[0037] The gel used for the content of organophosphorus and carbamate pesticides provided by the present embodiment comprises the following components and parts by volume: 90 parts of polyvinyl alcohol with a mass concentration of 2%, 90 parts of chitosan acetic acid solution, and a mass concentration of 2%. 2 parts of 37% formaldehyde, 8 parts of cholinesterase solution with enzyme activity of 0.2 U / ml. Its preparation method is identical with embodiment 1.

Embodiment 3

[0039] The gel used for the content of organophosphorus and carbamate pesticides provided by the present embodiment comprises the following components and parts by volume: 110 parts of polyvinyl alcohol with a mass concentration of 2%, 110 parts of chitosan acetic acid solution, and a mass concentration of 2%. 2 parts of 37% formaldehyde, 20 parts of cholinesterase solution with enzyme activity of 0.2 U / ml. Its preparation method is identical with embodiment 1.

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PUM

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Abstract

The invention discloses a gel for detecting content of organophosphorus and carbamate pesticide. The gel is prepared from polyvinyl alcohol, a chitosan acetic acid solution, formaldehyde with a mass concentration of 37 percent, and a cholinesterase solution with vitality of 0.2U / ml. Cccording to the principle that cholinesterase is used to catalyze the hydrolysis of indigo phenol acetate into acetic acid and indigo phenol, and the organophosphorus or carbamate pesticide can inhibit choline ester, enzyme-containing gel is prepared from polyvinyl alcohol (PVA), acetic acid, chitosan (CS), formaldehyde and acetylcholinesterase, so that the gel has very high water absorption. The gel contains acetylcholinase, so that the gel can be directly used for the detection of the content of organic phosphorus and carbamate agricultural pesticide. Moreover, the gel is used as an enzyme carrier, and is easy to store in a refrigerated container in order to ensure the enzyme activity and improve the accuracy of detection.

Description

technical field [0001] The invention relates to a gel for detecting the content of organic phosphorus and carbamate pesticides and an application thereof, belonging to the technical field of agricultural product safety detection. Background technique [0002] In recent years, food safety issues have seriously threatened human health, and the pesticide residues and acute and chronic poisoning in fruits and vegetables caused by excessive use or abuse of pesticides have attracted great attention from the society. At present, there are two main methods for rapid detection of whether the residues of organophosphorus and carbamate pesticides in fruits and vegetables exceed the standard: [0003] 1. Quick test card method, including red tablets (containing indophenol acetate) and white tablets (containing cholinesterase), first drop the solution to be tested on the white tablets (containing cholinesterase), and put Superimpose the white tablet and the red tablet together, and obse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38G01N21/78G01N21/31
CPCG01N1/38G01N21/3103G01N21/78
Inventor 王维静张靖新黎威志李方圆张希仁游延军
Owner 四川众益生物科技有限公司
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