Cell preparation reagent, kit and usage

A cell preparation and kit technology, applied in the field of cell preparation reagents, can solve problems such as pollution, increase products, etc., and achieve the effects of convenient operation, improved adhesion, and improved processing quality.

Pending Publication Date: 2018-12-18
MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, frequent access takes up a lot of precious time, and at the same time increases the probability of product contamination after opening

Method used

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  • Cell preparation reagent, kit and usage
  • Cell preparation reagent, kit and usage
  • Cell preparation reagent, kit and usage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042]

[0043]

[0044] Prepare 100g of sample processing reagent according to the above components, dissolve the above components in ultrapure water, stir and mix, and make up 100g of ultrapure water.

[0045] Packing process: Take a 10ml quantitative dispenser, quantitatively dispense 2ml of the sample processing reagent into the tube body of the cell production kit, and seal it with a sealed cover. 5 pieces are used as a group, and they are plastic-sealed in a blister box. into merchandise sales.

[0046] Instructions:

[0047] Step 1: Remove the sealing cap, add the sample, and re-fix the sealing cap on the reagent storage tube;

[0048] Step 2: Place the kit on the cell smear centrifuge;

[0049] Step 3: centrifuge at 1500 rpm for 2 minutes, discard the supernatant;

[0050] Step 4: Take the slides and fix them in 95% ethanol.

[0051] According to the above-mentioned steps, the film production is completed, and Papanicolaou staining is performed, and the effec...

Embodiment 2

[0053]

[0054]

[0055] Prepare 100g of sample processing reagent according to the above components, dissolve the above components in ultrapure water, stir and mix, and make up 100g of ultrapure water.

[0056] Packing process: Take a 10ml quantitative dispenser, quantitatively dispense 2ml of the sample processing reagent into the tube body of the cell production kit, and seal it with a sealed cover. 10 pieces are used as a group, and they are plastic-sealed in a blister box. into merchandise sales.

[0057] Instructions:

[0058]Step 1: Remove the sealing cap, add the sample, and re-fix the sealing cap on the reagent storage tube;

[0059] Step 2: Place the kit on the cell smear centrifuge;

[0060] Step 3: centrifuge at 1100 rpm for 2 minutes, discard the supernatant;

[0061] Step 4: Take the slides and fix them in 95% ethanol.

[0062] According to the above-mentioned steps, the film production is completed, and Papanicolaou staining is performed, and the effec...

Embodiment 3

Make up 100g

[0065] Prepare 100g of sample processing reagent according to the above components, dissolve the above components in ultrapure water, stir and mix, and make up 100g of ultrapure water.

[0066] Packing process: Take a 10ml quantitative liquid dispenser, quantitatively dispense 2ml of sample processing reagent into the tube body of the tablet maker, and seal it with a sealing cover. 25 pieces are used as a group, and plastic-sealed in a blister box to make a product Sales.

[0067] Instructions:

[0068] Step 1: Remove the sealing cap, add the sample, and re-fix the sealing cap on the reagent storage tube;

[0069] Step 2: Place the kit on the cell smear centrifuge;

[0070] Step 3: Spin up the centrifuge at 1100 speed for 3 minutes, discard the supernatant;

[0071] Step 4: Take the slides and fix them in 95% ethanol.

[0072] According to the above-mentioned steps, the film production is completed, and Papanicolaou staining is performed, and the effe...

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PUM

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Abstract

The invention relates to a cell preparation kit. The kit comprises a reagent shell storing a reagent, a glass slide and a mobile fixed plate, wherein the reagent shell comprises a tube body accommodating the reagent, a cavity accommodating the glass side and the mobile fixed plate, and a sealing ring further sealing the cavity and the glass slide, the glass slide is loaded in the cavity, then themobile fixed plate is loaded in the cavity so as to fix the glass slide. According to the kit, a sample processing reagent is loaded in a reagent storage part in advance, the sample processing reagentmeets client experiment demand volume and is characterized by being a part of a cell preparation kit integrating transportation, storage and processing, so that the client can take and use the sampleprocessing reagent conveniently, the sample is less likely to contaminate, social resources are saved, impurities in the sample are removed effectively, interference is avoided, and a pathologist canbetter review the glass slide.

Description

technical field [0001] The invention relates to a pathological testing instrument and reagent, in particular to a cell slice preparation reagent, a cell slice preparation kit and a use method. Background technique [0002] Isolation and purification of diagnostic components from clinical samples is a basic method in many biological and medical applications, and it is widely used in fields including cervical cytology examination and early diagnosis of viral infection in bone marrow transplant patients. It is not only the first step in sample processing, but also a crucial step, because the confidence of subsequent diagnostic results depends largely on the validity and purity of the isolated components. [0003] Isopycnic sedimentation is used to separate particles of unequal density. Cells or organelles settle or float to a medium with the same density as their own after sufficient centrifugal force and long enough time in the medium, and stay there to achieve equilibrium, t...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/2813
Inventor 黄荣祥朱晨雁
Owner MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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