A compound oligopeptide for improving sexual function and anti-fatigue, its oral preparation and the preparation method of the oral preparation
An oral preparation and oligopeptide technology, which is applied to the oral preparation and the preparation of the oral preparation, and the field of compound oligopeptide, can solve the problems of unclear side effects, safety to be considered, etc.
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preparation Embodiment 1
[0069] (1) Shelling and beating: take fresh oysters, shell them to get oyster meat, add water, the mass ratio of water to oyster meat is 1:1, and beat into a slurry;
[0070](2) Enzymolysis treatment: adopt biological enzymolysis technology to carry out enzymolysis treatment to the slurry obtained in step (1), the enzyme used is alkaline protease (2.4AU / g, Novozymes, Denmark), and the amount of enzyme added is 1 / 2 of the oyster slurry quality. 2%, the enzymolysis temperature is 50°C, the pH is 8, and the enzymolysis time is 6 hours, the enzymolysis solution is obtained, and the degree of hydrolysis is 20.3%;
[0071] (3) Enzyme-inactivating treatment: the enzymatic hydrolyzate obtained in step (2) was subjected to boiling water to inactivate the enzyme for 10 minutes;
[0072] (4) Clarification treatment: the enzymolysis solution after step (3) deenzyme treatment adopts centrifugation to get the supernatant for subsequent use, centrifugation adopts 10000r / min, centrifugation 1...
preparation Embodiment 2
[0084] (1) Shelling and beating: take fresh oysters, shell them to get oyster meat, add water, the mass ratio of water and oyster meat is: 2:1, and beat into a slurry;
[0085] (2) Enzymolysis treatment: adopt biological enzymolysis technology to carry out enzymolysis treatment to the slurry obtained in step (1), the enzyme used is selected neutral protease (0.8AU / g, Novozymes, Denmark), and the amount of enzyme added is 1 / 2 of the substrate mass. 2%, the enzymolysis temperature is 40°C, the pH is 7, and the time is 8h, the enzymolysis solution is obtained, and the degree of hydrolysis is 19.4%;
[0086] (3) Enzyme-inactivating treatment: the enzymatic hydrolyzate obtained in step (2) was subjected to boiling water to inactivate the enzyme for 10 minutes;
[0087] (4) Clarification treatment: the enzymolyzed solution after step (3) deenzyme treatment adopts centrifugation to get the supernatant for later use, centrifugation adopts 15000r / min, and centrifuges for 10min;
[008...
preparation Embodiment 3
[0097] (1) Shelling and beating: take fresh oysters, shell them to get oyster meat, add water, the mass ratio of water and oyster meat is: 5:1, and beat into a slurry;
[0098] (2) Enzymolysis treatment: adopt biological enzymolysis technology to carry out enzymolysis treatment to the slurry obtained in step (1), the enzyme used is trypsin (1250USP / mg, Novozymes, Denmark), and the amount of enzyme added is 3% of the substrate mass , the enzymolysis temperature is 50°C, the pH is 7, and the time is 10 hours, the enzymolysis solution is obtained, and the degree of hydrolysis is 40.0%;
[0099] (3) Enzyme-inactivating treatment: the enzymatic hydrolyzate obtained in step (2) was subjected to boiling water to inactivate the enzyme for 10 minutes;
[0100] (4) Clarification treatment: the enzymolysis solution after step (3) deenzyme treatment adopts centrifugation to get the supernatant for subsequent use, centrifugation adopts 15000r / min, centrifugation 20min;
[0101] (5) separa...
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