Fermented morinda officinalis probiotics powder and preparation method thereof
A technology of Morinda officinalis powder and probiotics, which is applied in the food field and can solve problems such as the inability to realize the interaction between active ingredients of special substances and probiotics
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[0036] The invention provides a preparation method of fermented Morinda officinalis probiotic powder, comprising the following steps:
[0037] A) boiling the Morinda officinalis powder with water to obtain the processed Morinda officinalis powder;
[0038] B) inoculating the seed liquid of the probiotics into the fermentation medium to carry out fermentation culture to obtain the culture liquid;
[0039] The probiotics are selected from one or more of Lactobacillus plantarum, Bifidobacterium, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus paracasei, Bacillus subtilis, and Bacillus coagulans;
[0040] The fermentation medium comprises processed Morinda officinalis powder, glucose, salt and water;
[0041] C) The culture solution is mixed with soluble dietary fiber and oligosaccharides, freeze-dried, crushed and sieved to obtain fermented Morinda officinalis probiotic powder.
[0042] The present invention first processes Morinda officinalis powder, and concre...
Embodiment 1
[0074] (1) Treatment of Morinda officinalis
[0075] Scrub the freshly collected Morinda officinalis officinalis under running water until the surface is free of dirt and dust, remove the damaged part of the insect, dry at 60°C, remove the heart and cut it off, crush it with a pulverizer, and pass it through a 100-mesh sieve, according to the ratio of material to liquid Add boiled Morinda officinalis powder in water at a ratio of 1:2 until the water is absorbed dry, set aside;
[0076] (2) Preparation of probiotic strains and seed solution
[0077] The freeze-dried bacterial powders of Lactobacillus plantarum, Bifidobacterium, and Lactobacillus acidophilus were inoculated into MRS medium and activated for three generations, and the activated bacteria were cultured in MRS medium, and the bacteria were collected by centrifugation. Suspended with 0.85% physiological saline to prepare seed solution, the concentration of which was 1×10 8 CFU / mL;
[0078] (3) Preparation of ferme...
Embodiment 2
[0086] Concrete operation steps of the present invention are as follows:
[0087] (1) Treatment of Morinda officinalis
[0088]Scrub the freshly collected Morinda officinalis officinalis under running water until the surface is free of dirt and dust, remove the damaged part of the insect, dry at 60°C, remove the heart and cut it off, crush it with a pulverizer, and pass it through a 100-mesh sieve, according to the ratio of material to liquid Add boiled Morinda officinalis powder in water at a ratio of 1:2 until the water is absorbed dry, set aside;
[0089] (2) Preparation of probiotic strains and seed solution
[0090] The freeze-dried bacterial powders of Bifidobacterium, Lactobacillus paracasei, and Bacillus subtilis were transferred to MRS medium for activation for three generations, and the activated bacteria were cultured in MRS expansion medium, and the bacteria were collected by centrifugation. Finally, suspend with 0.85% physiological saline to prepare seed liquid,...
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