Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of active royal jelly oco polypeptide freeze-dried powder and preparation method thereof

A technology of royal jelly and freeze-dried powder, which is applied in the field of active royal jelly OCO polypeptide freeze-dried powder, preparation, royal jelly freeze-dried powder and its preparation field, which can solve the problems of OCO polypeptide products that have not been reported, so as to improve immunity and increase product yield , the effect of high reuse rate

Active Publication Date: 2021-09-07
广州百康细胞生命科技有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many studies on the enzymatic hydrolysis of royal jelly using pepsin, trypsin, animal protease, papain and other plant proteases, but there are still no reports on the use of animal proteases and plant proteases to enzymolyze royal jelly to obtain OCO polypeptide products and their applications.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of active royal jelly oco polypeptide freeze-dried powder and preparation method thereof
  • A kind of active royal jelly oco polypeptide freeze-dried powder and preparation method thereof
  • A kind of active royal jelly oco polypeptide freeze-dried powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A kind of active royal jelly OCO polypeptide freeze-dried powder is prepared by the following method:

[0042] 1) Material selection: select fresh royal jelly 5.0kg;

[0043] 2) Impurity removal: Add 5 times of water (25kg), stir and mix evenly, and then filter with 100-mesh filter cloth to remove foreign matter such as larvae and wax chips mixed into the royal jelly. Degree ≤ 20μm, spare;

[0044] 3) Enzymolysis:

[0045] One-time enzymatic hydrolysis: adjust the pH to 2.0, add pepsin with a mass ratio of 0.2%, mix well, and enzymolyze at a constant temperature of 37°C for 3 hours;

[0046] Secondary enzymatic hydrolysis: adjust the pH to 7.5, sequentially add trypsin and 0.4% papain with a mass ratio of royal jelly of 0.2%, mix well, and perform constant temperature enzymolysis at 37°C for 3 hours;

[0047] 4) Centrifugation, filtration and concentration: Centrifuge at 10000r / min, 2-4°C for 10-20min to obtain the supernatant; filter the microporous filtrate with a ...

Embodiment 2

[0055] A preparation method of active royal jelly OCO polypeptide freeze-dried powder is similar to Example 1, except that:

[0056] 1) Material selection: 5 kg of frozen royal jelly that maintains biological activity is selected;

[0057] 2) Impurity removal: add 3 times of water (15kg), stir and mix evenly, then filter with a 120-mesh filter cloth to remove foreign matter such as larvae and wax chips mixed in the royal jelly, and the filtrate is set aside;

[0058] 3) Protease immobilization step: inject sufficient amount of oxygen, immerse the substrate in dopamine alkaline solution for 6h-24h to form a modified substrate, and dry it for later use; Next, add a 1:1 protease solution, shake the shaker for immobilization, use ultrasonic assistance during the immobilization, remove the solution after completion, freeze-dry and set aside;

[0059] 4) Enzymatic hydrolysis: primary enzymatic hydrolysis: adjust the pH to 2.0, add 0.4% fabric-immobilized pepsin by mass, mix well, a...

Embodiment 3

[0062] Embodiment 3 (preparation, quality analysis and antioxidant experiment of royal jelly proteolysis active polypeptide)

[0063] For quality analysis and antioxidant experiment, present embodiment design 1# and 2# two comparative experiments, royal jelly main protein according to above-mentioned 1 respectively # ,2 # 、3 # Numbering is used to prepare royal jelly enzymatic polypeptide freeze-dried powder, and the experimental conditions are shown in Table 1:

[0064] Table 1 Enzyme hydrolysis experiment method of different enzymes

[0065]

[0066] 1 # ,2 # 、3 # The yield, relative molecular weight distribution, etc. of royal jelly polypeptide freeze-dried powder prepared by enzymatic hydrolysis experiment were tested and analyzed, and the results are shown in Table 2.

[0067] Table 2 Yield and relative molecular weight distribution of polypeptide freeze-dried powder obtained from enzymolysis experiments with different enzymes

[0068]

[0069] The above dete...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

When royal jelly protein is taken orally into the human body, it will be degraded into small molecule polypeptides by human pepsin, trypsin and other enzymes in the gastrointestinal tract, and then absorbed and utilized by the human body. To enhance its antioxidant properties, the present invention pre-treats royal jelly on the basis of the original royal jelly freeze-dried powder production technology, and performs combined enzymolysis on royal jelly protein through pepsin, trypsin and papain, so that complex high-molecular-weight proteins in royal jelly It is enzymatically hydrolyzed into small peptides with smaller and more uniform molecular weight, that is, nano-active oligopeptide collagen (OCO), which makes the product easier for the human body to absorb, and at the same time has stronger anti-oxidation, anti-aging, and immune-enhancing functions. Therefore, the method of the present invention is more beneficial to exert the nutritional function and health care function of royal jelly.

Description

technical field [0001] The invention belongs to the technical field of royal jelly deep processing, health food and beauty cosmetics, and relates to a royal jelly freeze-dried powder and a preparation method thereof, in particular to an active royal jelly OCO polypeptide freeze-dried powder, a preparation method and an application thereof. Background technique [0002] Royal jelly is secreted by the hypopharyngeal and mandibular glands of feeding bees, and is used to feed the queen bee and larvae of 1 to 3 days old. Royal jelly is rich in protein and has various biological functions such as immune regulation, anti-oxidation, anti-aging, anti-bacterial, anti-tumor and regulation of cardiovascular system. The research results of royal jelly show that the stability of the functional components of fresh royal jelly is positively correlated with the nutritional value and health care effect of royal jelly. The external performance of royal jelly quality and nutrition and health c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/14C12N11/00A23L33/18A61K8/64A61Q19/08
CPCA23V2002/00A61K8/64A61K2800/84A61Q19/08A23L33/18C07K1/14C12N11/00C12P21/06A23V2200/324A23V2200/302A23V2250/55
Inventor 陆群姚佳赖文军张全新
Owner 广州百康细胞生命科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com