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Lyophilized active royal jelly OCO polypeptide powder and preparation method thereof

A technology of royal jelly and freeze-dried powder, applied in the field of royal jelly freeze-dried powder and its preparation, active royal jelly OCO polypeptide freeze-dried powder, preparation field, can solve the problems that OCO polypeptide products have not been reported, and achieve easy human absorption and enhanced water solubility Effect

Active Publication Date: 2018-12-14
广州宙斯干细胞肽新材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many studies on the enzymatic hydrolysis of royal jelly using pepsin, trypsin, animal protease, papain and other plant proteases, but there are still no reports on the use of animal proteases and plant proteases to enzymolyze royal jelly to obtain OCO polypeptide products and their applications.

Method used

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  • Lyophilized active royal jelly OCO polypeptide powder and preparation method thereof
  • Lyophilized active royal jelly OCO polypeptide powder and preparation method thereof
  • Lyophilized active royal jelly OCO polypeptide powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A kind of active royal jelly OCO polypeptide freeze-dried powder is prepared by the following method:

[0042] 1) Material selection: select fresh royal jelly 5.0kg;

[0043] 2) Impurity removal: Add 5 times of water (25kg), stir and mix evenly, and then filter with 100-mesh filter cloth to remove foreign matter such as larvae and wax chips mixed into the royal jelly. Degree ≤ 20μm, spare;

[0044] 3) Enzymolysis:

[0045] One-time enzymatic hydrolysis: adjust the pH to 2.0, add pepsin with a mass ratio of 0.2%, mix well, and enzymolyze at a constant temperature of 37°C for 3 hours;

[0046] Secondary enzymatic hydrolysis: adjust the pH to 7.5, sequentially add trypsin and 0.4% papain with a mass ratio of royal jelly of 0.2%, mix well, and perform constant temperature enzymolysis at 37°C for 3 hours;

[0047] 4) Centrifugation, filtration and concentration: Centrifuge at 10000r / min, 2-4°C for 10-20min to obtain the supernatant; filter the microporous filtrate with a ...

Embodiment 2

[0055] A preparation method of active royal jelly OCO polypeptide freeze-dried powder is similar to Example 1, except that:

[0056] 1) Material selection: 5 kg of frozen royal jelly that maintains biological activity is selected;

[0057] 2) Impurity removal: add 3 times of water (15kg), stir and mix evenly, then filter with a 120-mesh filter cloth to remove foreign matter such as larvae and wax chips mixed in the royal jelly, and the filtrate is set aside;

[0058] 3) Protease immobilization step: inject sufficient amount of oxygen, immerse the substrate in dopamine alkaline solution for 6h-24h to form a modified substrate, and dry it for later use; Next, add a 1:1 protease solution, shake the shaker for immobilization, use ultrasonic assistance during the immobilization, remove the solution after completion, freeze-dry and set aside;

[0059] 4) Enzymatic hydrolysis: primary enzymatic hydrolysis: adjust the pH to 2.0, add 0.4% fabric-immobilized pepsin by mass, mix well, a...

Embodiment 3

[0062] Embodiment 3 (preparation, quality analysis and antioxidant experiment of royal jelly proteolysis active polypeptide)

[0063] For quality analysis and antioxidant experiment, present embodiment design 1# and 2# two comparative experiments, royal jelly main protein according to above-mentioned 1 respectively # ,2 # 、3 # Numbering is used to prepare royal jelly enzymatic polypeptide freeze-dried powder, and the experimental conditions are shown in Table 1:

[0064] Table 1 Enzyme hydrolysis experiment method of different enzymes

[0065]

[0066] 1 # ,2 # 、3 # The yield, relative molecular weight distribution, etc. of royal jelly polypeptide freeze-dried powder prepared by enzymatic hydrolysis experiment were tested and analyzed, and the results are shown in Table 2.

[0067] Table 2 Yield and relative molecular weight distribution of polypeptide freeze-dried powder obtained from enzymolysis experiments with different enzymes

[0068]

[0069] The above dete...

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Abstract

Royal jelly proteins are orally taken, enter a human body, and are degraded in the gastrointestinal tract by human pepsin, trypsin and other enzymes to form small molecular peptides, and the small molecular peptides are absorbed and utilized by the human body; royal jelly is preprocessed on the basis of an original lyophilized royal jelly powder production technology in order to well promote the absorption and utilization of the royal jelly by the human body, promote the royal jelly to quickly act and enhance the oxidation resistance of the royal jelly; and combined enzymatic hydrolysis of royal jelly proteins is carried out by pepsin, trypsin and papain to make complex high molecular weight proteins in the royal jelly enzymatically hydrolyzed into small peptides with small and uniform molecular weight, which are nanometer active oligopeptide collagen (OCO), so the product can be easily absorbed by the human body, and has strong oxidation resisting, aging resisting and immunity enhancing functions. The method facilitates the performance of the nutritional functions and the healthcare functions of the royal jelly.

Description

technical field [0001] The invention belongs to the technical field of royal jelly deep processing, health food and beauty cosmetics, and relates to a royal jelly freeze-dried powder and a preparation method thereof, in particular to an active royal jelly OCO polypeptide freeze-dried powder, a preparation method and an application thereof. Background technique [0002] Royal jelly is secreted by the hypopharyngeal and mandibular glands of feeding bees, and is used to feed the queen bee and larvae of 1 to 3 days old. Royal jelly is rich in protein and has various biological functions such as immune regulation, anti-oxidation, anti-aging, anti-bacterial, anti-tumor and regulation of cardiovascular system. The research results of royal jelly show that the stability of the functional components of fresh royal jelly is positively correlated with the nutritional value and health care effect of royal jelly. The external performance of royal jelly quality and nutrition and health c...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14C12N11/00A23L33/18A61K8/64A61Q19/08
CPCA23V2002/00A61K8/64A61K2800/84A61Q19/08A23L33/18C07K1/14C12N11/00C12P21/06A23V2200/324A23V2200/302A23V2250/55
Inventor 陆群姚佳赖文军张全新
Owner 广州宙斯干细胞肽新材料科技有限公司
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