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Vagina mist spray containing mesenchymal stem cell factor and preparing method thereof

A technology of stem cells and factors, applied in the field of cell biology and biomedicine, can solve the problems of preparation method and application that have not been reported, and achieve the effect of preventing vaginal atrophy

Inactive Publication Date: 2018-12-14
浙江因诺泰医疗器械有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, the clinical discomfort symptoms and the decline in the quality of life brought by vaginal tissue aging to women have not yet attracted sufficient attention in China, and the market for related products is relatively blank, especially the use of mesenchymal stem cells or their secreted factors as the main component for vaginal tissue anti-aging and There are no reports on the preparation method and application of products for aging damage repair

Method used

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  • Vagina mist spray containing mesenchymal stem cell factor and preparing method thereof
  • Vagina mist spray containing mesenchymal stem cell factor and preparing method thereof

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Embodiment 1

[0025] A vaginal spray containing mesenchymal stem cell factors and its preparation method. Take the umbilical cord tissue of healthy full-term normal delivery or cesarean section neonates, take it back to the clean cell room for processing within 6 hours, and remove the parts with pinch marks and congestion on both sides. The periphery of the umbilical cord and the lumen of the vein were fully rinsed with PBS containing double antibodies. Culture primary cells by tissue block method, cut longitudinally along the umbilical vein lumen, peel off the umbilical vein intima, and cut the tissue into 1-3mm 3 Size, paste the tissue block evenly and tightly (1mm spacing) on ​​the bottom of the culture bottle, add 1ml of complete medium (Gibco's DMEM / F12+10%FBS) and place it in a normal carbon dioxide incubator with the bottom wall facing up, turn the culture bottle over after 4 hours, Afterwards, 1ml of complete medium was added every 24 hours, and the medium was changed twice a week a...

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Abstract

Vagina mist spray containing a mesenchymal stem cell factor and a preparing method thereof are disclosed. The mist spray includes, by mass, 0.001-0.1% of a mesenchymal stem cell factor, 0.05-2% of sodium hyaluronate, 1-10% of oat glucan, 1-20% of glycerol, 0.1-0.5% of lactic acid, and 0.2-1.0% of sodium lactate, with the balance being purified water. A cytobiology manner is adopted, and repairingcapability of the stem cell factor is applied to effectively prevent vagina atrophy, to treat colpoxerosis and senile vaginitis caused by vagina atrophy, to delay male vagina tissue senility and to repair vagina tissue senility injuries.

Description

technical field [0001] The invention relates to the technical fields of cell biology and biomedicine, in particular to a vaginal spray containing mesenchymal stem cell factors and a preparation method thereof. Background technique [0002] Mesenchymal stem cells are a type of adult stem cells that exist in a variety of tissues, such as bone marrow, umbilical cord blood, and fat, and have multi-directional differentiation potential. Cell growth factors and insulin growth factors, etc., these factors secreted outside the cells can play an important role in supporting and regulating. [0003] Scientific research has found that mesenchymal stem cells have significant effects in the repair of human tissue aging damage, treatment of aging degenerative diseases or delaying the age-dependent function decline of tissues and organs, and have received support from a large number of clinical trials. Since the direct application of mesenchymal stem cells to the human body can increase t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K9/12A61P15/02A61P39/06
CPCA61K9/0034A61K9/12A61K38/18A61P15/02A61P39/06
Inventor 杨维童振飞陈跃义罗海浪徐丽君
Owner 浙江因诺泰医疗器械有限公司
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