Application of serum S100a8/9 complex level in diagnosis and prognosis of acute myocardial infarction
A technology of acute myocardial infarction and complex, applied in the field of medicine and biology, can solve the problem of not reflecting the characteristics of ischemia-reperfusion injury, etc.
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Embodiment 1
[0054] Example 1: Differences in S100a8 / 9 expression levels between AMI patients and healthy individuals
[0055] According to the principle of gender and age matching, serum samples of 207 healthy people and 208 AMI patients were selected, and the expression level of S100a8 / 9 was detected by ELISA experiment. The expression level of S100a8 / 9 in AMI patients was 1.91 times higher than that in healthy people (P<0.001).
[0056] 1. Experimental steps:
[0057] Kit: R&D Systems, Inc, Human S100A8 / S100A9, Heterodimer Immunoassay
[0058] (1) Reagent preparation:
[0059] 1. Equilibrate all reagents to room temperature before use.
[0060] 2. Wash Buffer: If crystals have formed in the concentrated solution, equilibrate to room temperature and shake gently until the crystals are completely dissolved, then add ionized water or distilled water to dilute 20ml of the wash buffer to 500ml.
[0061] 3. Substrate Solution: Chromogenic reagents A and B should be mixed in equal volumes ...
Embodiment 2
[0084] Example 2: The expression of S100a8 / 9 at two consecutive time points in 210 AMI patients before PCI and 1 day after PCI
[0085] 210 AMI patients PCI preoperative (0 day), postoperative 1 day (1 day), continuous 2 time point samples, detect the expression level of S100a8 / 9 protein complex in the patient's serum by the ELISA experiment identical with embodiment 1 .
[0086] 1. The detection results of the expression level of S100a8 / 9 at 1 day after operation are shown in figure 2 , Table 2, the results showed that the expression level of S100a8 / 9 after PCI was higher than that before PCI (P<0.001).
[0087] Table 2. Serum S100a8 / 9 protein complex content of patients before and after PCI
[0088]
Embodiment 3
[0089] Example 3: Prognostic value of S100a8 / 9 on in-hospital MACE events
[0090] According to the occurrence of MACE events (death, acute heart failure) in the hospital, 210 AMI patients were divided into MACE group (35 people) and Non-MACE group (175 people). The expression level of the S100a8 / 9 protein complex in the patient's serum was detected by the same ELISA experiment as in Example 1.
[0091] Statistics show:
[0092] ① Differences in the expression of the S100a8 / 9 complex between the MACE group and the Non-MACE group before PCI (0 day) and 1 day after PCI (1 day). At 1 day after operation, there was a significant statistical difference between the two groups, and the MACE group was 1.57 times higher than the Non-MACE group (P image 3 and Table 3.
[0093] Table 3. Serum S100a8 / 9 protein complex content of patients in MACE group and Non-MACE group before and after PCI
[0094]
[0095] ②A logistic regression model was established to illustrate the relationship...
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