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Preparation method and application of amperometric immunosensor capable of realizing mutual verification by using dual detection method

An immune sensor and current-type technology, applied in nanotechnology for sensing, nanotechnology for materials and surface science, instruments, etc., can solve the problem that the detection method cannot explain the accuracy of the sensor, and achieve accurate quantification The effect of detection of carcinoembryonic antigen, large surface area, and improved sensitivity

Inactive Publication Date: 2018-12-11
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A single detection method cannot account for the accuracy of the constructed sensor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Preparation of Au@Pd NDs / Fe 2+ -CS / PPy NTs / Ab 2 include:

[0047] ① Preparation of gold-palladium core-shell nanodendrites (Au@Pd NDs)

[0048] Take 0.4 mL, 1.0 wt% chloroauric acid solution and add it to 49 mL of secondary water, heat it to boiling, quickly add 1.0 mL, 1.0 wt% sodium citrate solution, and keep boiling for 10 min under stirring conditions to obtain gold nanoparticles Particle dispersion: Add 80 mg of hexadecylpyridinium chloride, 8.0 mL of secondary water and 0.5 mL, 20 mmol / L palladium chloride solution to 15 mL of gold nanoparticle dispersion, stir for 10 min and then add 1.0 mL, 0.1 mol / L freshly prepared ascorbic acid solution, placed at 40°C for 4 h, centrifuged, washed with water, and freeze-dried to obtain Au@Pd NDs;

[0049] ② Preparation of chitosan-functionalized polypyrrole nanotubes (CS / PPy NTs)

[0050] Take 0.2 g of ferric chloride and disperse it in 12 mL of secondary water, add 12 mL of 10 mmol / L methyl orange solution unde...

Embodiment 2

[0055] Example 2 Preparation of Au@Pd NDs / Fe 2+ -CS / PPy NTs / Ab 2 include:

[0056] ① Preparation of gold-palladium core-shell nanodendrites (Au@Pd NDs)

[0057] Take 0.5 mL, 1.0 wt% chloroauric acid solution and add it to 49 mL of secondary water, heat to boiling, quickly add 1.3 mL, 1.0 wt% sodium citrate solution, and keep boiling for 10 min under stirring conditions to obtain gold nanoparticles Particle dispersion: Add 85 mg of hexadecylpyridinium chloride, 8.0 mL of secondary water and 0.8 mL of 20 mmol / L palladium chloride solution to 18 mL of gold nanoparticle dispersion, stir for 10 min and then add 1.3 mL, 0.1 mol / L freshly prepared ascorbic acid solution, placed at 40°C for 4 h, centrifuged, washed with water, and freeze-dried to obtain Au@Pd NDs;

[0058] ② Preparation of chitosan-functionalized polypyrrole nanotubes (CS / PPy NTs)

[0059] Take 0.25 g of ferric chloride and disperse it in 14 mL of secondary water, add 13 mL of 10 mmol / L methyl orange solution unde...

Embodiment 3

[0064] Example 3 Preparation of Au@Pd NDs / Fe 2+ -CS / PPy NTs / Ab 2 include:

[0065] ① Preparation of gold-palladium core-shell nanodendrites (Au@Pd NDs)

[0066] Take 0.6 mL, 1.0 wt% chloroauric acid solution and add it to 49 mL of secondary water, heat to boiling, quickly add 1.5 mL, 1.0 wt% sodium citrate solution, and keep boiling for 10 min under stirring conditions to obtain gold nanoparticles Particle dispersion: Add 90 mg of hexadecylpyridinium chloride, 8.0 mL of secondary water and 1.0 mL of 20 mmol / L palladium chloride solution to 20 mL of gold nanoparticle dispersion, stir for 10 min and then add 1.5 mL, 0.1 mol / L freshly prepared ascorbic acid solution, placed at 40°C for 4 h, centrifuged, washed with water, and freeze-dried to obtain Au@Pd NDs;

[0067] ② Preparation of chitosan-functionalized polypyrrole nanotubes (CS / PPy NTs)

[0068] Take 0.3 g of ferric chloride and disperse it in 15 mL of secondary water, add 15 mL of 10 mmol / L methyl orange solution under...

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Abstract

The invention belongs to the technical field of novel nano materials, immunoassay and bio-sensing, and provides a preparation method and application of an amperometric immunosensor capable of realizing mutual verification by using a dual detection method. According to the preparation method, the sandwich type amperometric immunosensor is constructed by taking gold nanoparticle-loaded aminated graphene (Au NPs / NH2-GS) as a substrate platform and a gold-palladium core-shell nano dendrite-loaded Fe<2+>-absorbed chitosan functional polypyrrole nanotube composite material (Au@Pd NDs / Fe<2+>-CS / PPy NTs) as a marker. The constructed immunosensor detects carcino-embryonic antigens with different concentrations by using a time-current curve method and a square-wave pulse voltammetry method, the coincident linear parts of the two test results are mutually verified, so that the accuracy of the sensor can be improved, and a reliable detection means is provided for accurately detecting the carcino-embryonic antigens.

Description

technical field [0001] The invention belongs to the technical fields of novel nanomaterials, immune analysis and biosensing, and provides a preparation method and application of a current-type immune sensor that utilizes dual detection methods to verify each other, and specifically relates to a gold-palladium-based core-shell nanodendrite Adsorption of Fe 2+ The chitosan-functionalized polypyrrole nanotube composite material is used to construct an amperometric immunosensor, and the application of the immunosensor in the detection of carcinoembryonic antigen by time-current curve method and square wave pulse voltammetry. Background technique [0002] According to the report of the World Health Organization in February 2018, about 8.8 million people died of cancer in the world in 2015. Chen Wanqing and others also mentioned in the article "Cancer statistics in China, 2015" that the total number of new cancer cases in China in 2015 is expected to be 4.292 million, and the exp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N33/574B82Y30/00B82Y15/00
CPCB82Y15/00B82Y30/00G01N27/3277G01N27/3278G01N33/57473G01N2800/7028
Inventor 王平裴福彬马恩慧李明党余昊轩薛建强王瑞琛董云会李月云
Owner SHANDONG UNIV OF TECH
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