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A kind of semi-open bed type agricultural fungal microorganism culture method

A cultivation method and microbial technology, which is applied in the field of agricultural fungal microorganism cultivation, can solve the problems of discarded cultivation process, high investment cost, long fermentation time, etc., and achieve the effect of simple operation, short construction period and simple equipment

Active Publication Date: 2022-08-02
山东沃尔美肥业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of steaming ball culture is often used in the cultivation of agricultural fungi microorganisms. When this method is used for the cultivation of agricultural fungi microorganisms, the fermentation time is generally longer, usually about 5-7 days, and the whole cultivation process The equipment used is various, the input cost is high, the energy consumption is high, but the production capacity is severely limited, and the cultivation environment is relatively strict during the cultivation process using traditional methods. Once pollution occurs, the entire cultivation process will be destroyed. scrapped, so the existing method of cultivating agricultural fungi and microorganisms using steaming balls and other equipment cannot well meet the needs of the market

Method used

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  • A kind of semi-open bed type agricultural fungal microorganism culture method
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for culturing a semi-open bed type agricultural fungal microorganism, the specific steps comprising:

[0034] S1: Medium treatment: add water to the medium to a water content of 55%, and naturally ferment the medium. When the temperature rises to above 60°C, keep it for 3 days. Under this condition, most of the bacteria in the medium will Death, leaving only fibromycetes, hemifibrils, actinomycetes, etc., which can withstand high temperature.

[0035] When the medium is spread out on the culture bed, the temperature of the medium will drop to 28°C. At this time, the fibromycetes, hemicelluloses, actinomycetes, etc., which can withstand high temperature in the medium, have stopped growing, and can be heated at 28°C. C. The normal growth of miscellaneous bacteria has been killed in the high temperature fermentation in the previous stage. At this time, the inoculation of agricultural fungal strains is suitable for its growth. In the case of the advantage of agric...

Embodiment 2

[0052] A method for culturing a semi-open bed type agricultural fungal microorganism, the specific steps comprising:

[0053] S1: Medium treatment: add water to the medium to a water content of 60%, and naturally ferment the medium. When the temperature rises above 60°C, keep it for 3 days. Under these conditions, most of the bacteria in the medium will Death, leaving only fibromycetes, hemifibrils, actinomycetes, etc., which can withstand high temperature.

[0054] When the medium is to be spread out on the cultivation bed, the temperature of the medium will be lowered to 35°C. At this time, the fibrous bacteria, hemicellulose, actinomycetes, etc., which can withstand high temperature in the medium, have stopped growing, and can be heated at 35°C. C. The normal growth of miscellaneous bacteria has been killed in the high temperature fermentation in the previous stage. At this time, the inoculation of agricultural fungal strains is suitable for its growth. In the case of the ...

Embodiment 3

[0070] A method for culturing a semi-open bed type agricultural fungal microorganism, the specific steps comprising:

[0071] S1: Medium treatment: add water to the medium to a water content of 65%, and naturally ferment the medium. When the temperature rises to above 60°C, keep it for 3 days. Under this condition, most of the bacteria in the medium will Death, leaving only fibromycetes, hemifibrils, actinomycetes, etc., which can withstand high temperature.

[0072] When the medium is to be spread out on the cultivating bed, the temperature of the medium will drop to 30°C. At this time, the high-temperature-resistant fibromycetes, hemicelluloses, and actinomycetes in the medium have stopped growing, and can be heated at 30°C. C. The normal growth of miscellaneous bacteria has been killed in the high temperature fermentation in the previous stage. At this time, the inoculation of agricultural fungal strains is suitable for its growth. In the case of the advantage of agricultu...

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Abstract

The invention relates to a method for culturing agricultural fungal microorganisms, in particular to a method for culturing a semi-open bed type agricultural fungal microorganism. The specific steps include: culture medium treatment: adding water to the culture medium to a water content of 55-65%, naturally fermenting, and waiting for When the temperature rose to more than 60 ° C, kept for 3 days; bed making: the fermented medium was spread on the culturing device; Inoculation: inoculate by 3-5% of the medium weight, and cover it with a seed after the seed inoculation is completed. layer of plastic film; fermentation culture: the filtered air is filled into the culture medium, and the temperature is maintained at 28-35°C for 5--8 days; 55%, water can be replenished to 60%; production of finished products: After the cultivation is completed, the medium is continuously turned, and the ventilation volume is increased to promote the rapid water loss of the medium to 15%-25%. After the medium is mixed, the viable bacteria content is detected. After reaching the standard, the finished product is packaged and put into storage.

Description

technical field [0001] The invention relates to a method for culturing agricultural fungal microorganisms, in particular to a method for culturing agricultural fungal microorganisms in a semi-open bed. Background technique [0002] The steaming ball is a rotary batch cooking and pulping equipment. It is spherical, mainly composed of a sphere, a machine base, a transmission device, etc. The sphere is made of steel plates welded or riveted; a charging hole is opened at the vertical centerline of the sphere for charging and feeding into the cooking liquid. The journal is fed with steam for cooking and heating and discharged with condensed water. At present, the method of steaming ball culture is often used in the process of culturing agricultural fungi microorganisms. When using this method to cultivate agricultural fungi microorganisms, the fermentation time is generally longer, usually about 5-7 days, and the whole process of cultivation. The equipment used is diverse, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
CPCC12N1/14
Inventor 周长青殷碧秋赵学臣任庆良蔡红星王坤
Owner 山东沃尔美肥业有限公司
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