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Salt-resistant and alkali-resistant soil aspergillus strain as well as ITS sequence and application thereof

A technology of Aspergillus terreus and alkali resistance, applied in application, fungi, and microbial-based methods, etc., can solve problems such as difficult development, activity inhibition, and limited microbial species, and achieve the effect of broad application prospects

Active Publication Date: 2018-12-07
辽宁伊品九利农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the types of microorganisms with salt and alkali resistance are limited. At the same time, the activity of microorganisms that can purify sewage and decompose agricultural waste in normal environments is greatly inhibited in high-saline land, and it is difficult to play their role.

Method used

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  • Salt-resistant and alkali-resistant soil aspergillus strain as well as ITS sequence and application thereof
  • Salt-resistant and alkali-resistant soil aspergillus strain as well as ITS sequence and application thereof
  • Salt-resistant and alkali-resistant soil aspergillus strain as well as ITS sequence and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation of Aspergillus flocculus JYJ-5 Strain

[0028] Isolation of the strains: The soil samples for the isolation of the strains were from Changchun City, Jilin Province. The five-point sampling method was used to collect samples at the collection site, and the soil within 30 cm deep of the soil plow layer was taken, put into sterilized glass bottles and brought back to the laboratory for later use. Using the dilution coating method, the diluted and clarified soil supernatant was spread on the potato dextrose agar medium (PDA) supplemented with NaCl to 150.0g / L, cultivated for 4 days under the condition of pH=10, and passed through 3 rounds of screening A fungal strain resistant to salt and alkali, Aspergillus terreus JYJ-5, was isolated. This strain was deposited in the General Microorganism Center of China Committee for the Collection of Microbial Cultures on March 19, 2018, with the preservation number CGMCC 15368.

Embodiment 2

[0030] Identification of Aspergillus flocculus JYJ-5 Strain

[0031] 1. Morphological identification of the bacterial strain: the Aspergillus terreus JYJ-5 screened in Example 1 was inoculated onto Chase agar medium, cultured at 30° C. for one week, the colony morphology was observed and its sporulation structure and hyphae morphology were examined under a microscope. After being cultured on a Chase agar plate at a temperature of 30°C for 1 week, the colony is round or nearly round with a raised center, such as figure 1 shown. Use a pick needle to take the mycelia on the surface layer of the colony, make a glass slide, and carry out microscopic observation: the mycelia and spore peduncles are smooth, the conidiophores grow upright, the tops of the stalks are enlarged and spherical, and conidia germinate on the top of the small stalks, as shown in Fig. image 3 and Figure 4 shown. The conidia are spherical unit cells, such as figure 2 shown. Morphologically identified as...

Embodiment 3

[0035] Identification of Salt and Alkali Resistance and Optimum Salt Concentration of Aspergillus terreus JYJ-5

[0036] 1. the mensuration of Aspergillus terreus JYJ-5 optimum salt concentration: the PDA medium of preparation gradient NaCl content, the pH of described medium is 7.0, and its salt concentration increases to 31wt% (saturated concentration) by 1wt%, every Level increased by 2%, with no NaCl added PDA as the control group. An equal amount of strains were inoculated in the center of the culture medium and cultured at 30°C for 1 week. It was found that the strains could grow in 0%-31% NaClPDA medium at a temperature of 30°C, but the growth of the strains was significantly inhibited as the salt concentration increased. The growth is relatively slow at the concentration of 1%-5%; the growth of the strain is accelerated at the concentration of 5%-15%, the growth of the strain is sharply slowed at the concentration of 17%-25%, and the bacteria basically do not grow at ...

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Abstract

The invention provides a salt-resistant and alkali-resistant soil aspergillus strain as well as an ITS sequence and application thereof. The preservation name of the soil aspergillus strain is a flocculent soil aspergillus JYJ-5 strain, with the preservation number of CGMCC 15368. The strain has the salt-resistant and alkali-resistant properties, and can be grown preferably in a sodium salt environment and an alkali environment for a long time, and specially can be grown preferably under a high salt condition of sodium salt concentration of 5 percent to 15 percent (gram / milliliter) and an alkali condition of pH of 7 to 12. The strain provided by the invention has wide application prospects in reconstruction of saline-alkali land.

Description

technical field [0001] The invention belongs to the field of microbial engineering and technology, and specifically relates to a salt-and-alkali-resistant Aspergillus terreus strain, its ITS sequence and application. Background technique [0002] With the rapid development of the economy, environmental problems have become increasingly severe, such as land salinization, agricultural waste pollution, and sludge sewage pollution. [0003] The research on the improvement of saline-alkali land has a history of nearly a hundred years. Generally speaking, the improvement of saline-alkali land is mainly through water conservancy improvement, agricultural technology improvement, biological improvement, chemical improvement and other methods. Water conservancy improvement requires the establishment of a complete drainage and irrigation system; agricultural improvement mainly enhances soil permeability and accelerates salt leaching through deep plowing, leveling the land, and adding o...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/11C12Q1/6895A01B79/00C12R1/66
CPCA01B79/00C12N1/14C12Q1/6895C12R2001/66C12N1/145
Inventor 史洋张森林张子宇朱赤
Owner 辽宁伊品九利农业科技发展有限公司
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