Immunomagnetic bead labeling-based SERS sorting method

Abstract

The invention provides an immunomagnetic bead labeling-based SERS sorting method. The method comprises the following steps: S1, labeling the surface receptor specific to each target cell with an immunomagnetic bead modified with an antibody paired with the surface receptor; S2, screening out the target cells; S3, labeling each target cell with SERS-TAG modified with the antibody paired with the surface receptor according to the surface receptor specific to each target cell; and S4, carrying out surface enhanced Raman detection on each labeled target cell to determine the types of the target cells, and classifying the target cells according to the types of the target cells. The method has the advantages of completion of multiple CTC detections in one test, great reduction of the frequency of surface enhanced Raman detection and the use amount of the SERS-TAG, shortening of the optical detection time, and cost saving.

Description

technical field [0001] The invention belongs to the cross technical field of cell sorting and optical engineering, and in particular relates to a SERS sorting method based on immunomagnetic bead labeling. Background technique [0002] Immunomagnetic beads have been widely used in molecular and cell biology, including nucleic acid extraction, sorting and enrichment of specific proteins and cells, etc. Taking immunomagnetic bead-based cell sorting as an example, the process is divided into four steps: (1) surface modification of magnetic beads with specific ligands or antibodies; (2) passing the modified magnetic beads through antigen- The antibody reaction or the ligand-receptor binding reaction binds to the target cells in the sample; (3) magnetically adsorbs the magnetic bead-cell conjugate; (4) removes other cells by washing and other means. [0003] The existing cell screening technology based on immunomagnetic beads directly attracts magnetic beads through magnetic forc...

AI Technical Summary

Problems solved by technology

This protocol can remove other cells and unbound magnetic beads, but it is difficult to ensure that trace target cells are successfully captured

Moreover, this solution uses a chemical combination method, which is difficult to ensure the successful capture of very small cells such as circulating tumor cells, which will cause misdiagnosis in diagnosis

In addition, this method of capturing target cells through antibody-antigen binding requires manual participation and must be completed by professional technicians, which is not conducive to integration and cannot meet the informatization needs of the big data era

[0006] In addition, immunomagnetic beads are used to mark specific antigens or receptors on target cells. This method can only sort out one type of target cells under certain circumstances, and it is impossible to confirm whether other types of tumor cells are contained in whole blood samples.

However, in practical applications, for example, when a whole blood sample is used for tumor cell detection, only one kind of tumor cell is isolated, which is not enough to confirm whether the whole blood sample also contains other types of tumor cells, and cannot be further used for the detection of tumor cells (such as DNA sequencing, etc.) to provide accurate test sample information

At the same time, the same antigen or receptor may also exist on different tumor cells, and a variety of tumor cells can be sorted out at one time after being labeled with immunomagnetic beads. Accurate detection of sample information, thereby saving subsequent detection cycles and costs

However, the direct use of SERS detection requires the use of a large number of SERS-TAGs for labeling, resulting in waste, and the time to pass through the optical system is very long and the efficiency is extremely low

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