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Molecular markers and method for identifying Dimocarpus and related genera plants thereof

A technology of molecular markers and closely related genera, applied in the field of classification and identification of plant germplasm resources, to achieve the effect of simple operation and neat banding

Active Publication Date: 2018-11-30
POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI
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Problems solved by technology

So far, there have been no literature reports or patent applications using longan SSR markers to identify and distinguish Longan and its related genera

Method used

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  • Molecular markers and method for identifying Dimocarpus and related genera plants thereof
  • Molecular markers and method for identifying Dimocarpus and related genera plants thereof
  • Molecular markers and method for identifying Dimocarpus and related genera plants thereof

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Embodiment 1

[0039] Molecular markers and methods for identifying and distinguishing plants of the genus Longan and its closely related genera, using five genera of plants of the genus Longan, Sapindaceae, Shaozi, Litchi and Longli in Sapindaceae (Sapindaceae) example.

[0040] 1. experiment material

[0041]A total of 55 germplasm resources of Sapindaceae (Sapindaceae) were used in the study, including 24 samples of Longan genus Longan, 13 samples of Sapindaceae genus Sapindaceae, 2 samples of Shaozi rambutan, 8 samples of Litchi genus, Longli belongs to the 8 species of Longli, as shown in Table 2. The germplasm materials of longan and longli were collected from the National Fruit Tree Germplasm Fuzhou Longan Nursery, the litchi germplasm materials were collected from the Breeding Garden of the Fruit Tree Research Institute of Fujian Academy of Agricultural Sciences, and the sapindus germplasm materials were collected from Yangyan Road, Xindian Town, Jin'an District, Fuzhou City. Sagi...

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Abstract

The invention discloses molecular markers and a method for identifying Dimocarpus and related genera plants thereof, and belongs to the technical field of classification and identification of plant germplasm resources. According to the present invention, the genomic DNA of the Dimocarpus and the related genera plant are extracted by using the longan EST-SSR marker as the molecular marker, PCR amplification is performed with 9 pairs of universal EST-SSR primers, the amplified product is subjected to polyacrylamide gel electrophoresis, the spectral bands are read, the 0 / 1 data matrix is established, the genetic similarity coefficient is calculated, and cluster analysis is performed. According to the present invention, the universal EST-SSR primers compensate for the lack of the universal SSRmarkers in the Sapindaceae, and provides the effective markers for the classification research on the plant in the Sapindaceae; and the provided EST-SSR markers can accurately and effectively distinguish the Dimocarpus and the four related genera plants thereof, and has advantages of high repeatability, neat banding pattern, simple operation and the like.

Description

technical field [0001] The invention belongs to the technical field of classification and identification of plant germplasm resources, and specifically relates to a molecular marker and a method for identifying and distinguishing plants of the genus Longan and its close relatives. Background technique [0002] There are about 150 genera and 2,000 species of Sapindaceae plants (Luo Xianrui et al., 1985), and many species have important economic values ​​such as timber, food, medicine and energy supply. For a long time, plant taxonomists have disagreed on the definition of Sapindaceae and the location of taxa within the family. For example, there are two subfamilies (Judd S et al., 1994), four subfamilies (Umadevi I et al., 1991), four different subfamilies (Harrington MG et al., 2005), and five subfamilies (Thorne RF et al., 2000). Most of these classification viewpoints are based on morphology, palynology, fossil records and chemical composition, which requires high experie...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 胡文舜郑少泉陈秀萍
Owner POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI
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