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Fermentation culture medium and method for producing astaxanthin through fermentation of Phaffia rhodozyma

A technology of Phaffia rhodozyma and fermentation medium, which is applied in the field of fermentation medium for producing astaxanthin by fermentation of Phaffia rhodozyma, can solve problems such as low fermentation yield, achieve broad application prospects, inhibit tumor occurrence, and resist oxidation. powerful effect

Inactive Publication Date: 2018-11-27
WEIHAI LIDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing Phaffia rhodozyme fermentation yields are generally low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for fermenting high-yield natural astaxanthin by using Phaffia red yeast, and its technical scheme is:

[0025] (1) The bacteria required for the experiment are activated in seed culture for 3 generations, and shake culture in a shaker;

[0026] (2) Make sure that the fermentation medium contains glucose 100g / L (25g / L at the beginning of fermentation, and 75g / L at the end of fermentation), 5g / L yeast extract powder, 5g / L malt extract powder, 6g / L peptone, and sulfuric acid Ammonium 5 g / L, magnesium sulfate 0.5g / L, tomato powder 10g / L (5g / L at the beginning of fermentation, 5g / L at the later stage), 0.2% anhydrous ethanol (0.1% at the beginning of fermentation, 0.1% at the later stage) %).

[0027] (3) Use a 5L fermentor to carry out a fermentation experiment. Inoculate the cultivated tertiary seeds into a 5L fermentor, with an inoculum volume of 6%, a liquid volume of 3L, pH 5.0~5.5, temperature 20~22℃, low light culture , The stirring rate is 200~350rpm.

[0028] Th...

Embodiment 2

[0033] A method for fermenting high-yield natural astaxanthin by using Phaffia red yeast, and its technical scheme is:

[0034] (1) The bacteria required for the experiment are activated in seed culture for 3 generations, and shake culture in a shaker;

[0035] (2) Make sure that the fermentation medium contains glucose 120g / L (45g / L at the beginning of fermentation and 75g / L at the end of fermentation), 8g / L yeast extract powder, 8g / L malt extract powder, peptone 10g / L, sulfuric acid Ammonium 9g / L, magnesium sulfate 2.5g / L, tomato powder 10g / L (5g / L at the beginning of fermentation, 5g / L at the later stage), 0.5% of absolute ethanol (0.2% at the beginning of fermentation, 0.3% at the later stage).

[0036] (3) Use a 5L fermentor to carry out a fermentation experiment. Inoculate the cultivated tertiary seeds into a 5L fermenter, with an inoculum volume of 10%, a liquid volume of 3.5L, pH 5.0~5.5, temperature 20~22℃, low light Cultivate with a stirring rate of 200-350 rpm.

[0037] Th...

Embodiment 3

[0042] A method for fermenting high-yield natural astaxanthin by using Phaffia red yeast, and its technical scheme is:

[0043] (1) The bacteria required for the experiment are activated in seed culture for 3 generations, and shake culture in a shaker;

[0044] (2) Confirm that the fermentation medium contains glucose 140g / L (60g / L at the beginning of fermentation, 80g / L in the later stage of fermentation), 8g / L yeast extract powder, 8g / L malt extract powder, peptone 10g / L, sulfuric acid Ammonium 9g / L, magnesium sulfate 2.5g / L, tomato powder 10g / L (5g / L at the beginning of fermentation and 5g / L at the later stage), 0.5% of absolute ethanol (0.2% at the beginning of fermentation, 0.3% at the later stage).

[0045] (3) Use a 5L fermentor to carry out a fermentation experiment. Inoculate the cultivated tertiary seeds into a 5L fermenter, with an inoculum volume of 10%, a liquid volume of 3.5L, pH 5.0~5.5, temperature 20~22℃, low light Cultivate with a stirring rate of 200-350 rpm.

[00...

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Abstract

The invention discloses a fermentation culture medium and method for producing astaxanthin through fermentation of Phaffia rhodozyma. Each 1L of water in the fermentation culture medium contains the following components by weight: 80g-140g of glucose, 5g-8g of yeast extract powder, 5g-8g of malt extract powder, 6g-10g of proteins, 5g-9g of ammonium sulfate, 0.5g-2.5g of magnesium sulfate, 10g-30gof tomato powder and 0.2%-0.5% (volume fraction) of absolute ethyl alcohol. The invention discloses the culture medium and method for highly producing astaxanthin by virtue of Phaffia rhodozyma. By carrying out fermentation through the method, the biomass of thalli and the content of astaxanthin are high, the oxidation resistance of astaxanthin is strong and is equal to 550 times of that of vitamin E and 10 times of that of beta-carotene. Astaxanthin has physiological effects of enhancing the immunity, inhibiting tumors, eliminating in-vivo free radicals and the like and has wide application prospects in health products, medicines, cosmetics and the like.

Description

Technical field [0001] The present invention relates to the field of biological fermentation, in particular to a fermentation medium and method for producing astaxanthin by Phaffia red yeast. Background technique [0002] There are two methods for the production of astaxanthin: chemical synthesis and biosynthesis. Although the process of chemical production of astaxanthin is mature, astaxanthin produced by chemical method is completely different from natural astaxanthin in terms of structure, function and safety. In addition, the process is complicated, the reaction conditions are difficult to control, the production cost is high, and the safety limitations are high. Therefore, the US FDA has explicitly prohibited chemically synthesized astaxanthin from entering the health care product market. [0003] Natural astaxanthin has the advantages of safety, small side effects, and high biological activity. It has been approved by most countries for use in pharmaceutical, food and feed in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12N1/16C12R1/645
CPCC12N1/16C12P23/00
Inventor 李纪丰石英杰孙常明陈今朝赵佳蒋海燕足立胜
Owner WEIHAI LIDA BIOTECH
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