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Microbial liquid fungicide containing pantoea alhagi and preparation method and application of microbial liquid fungicide

A liquid bacterial agent and microbial technology, which is applied in the field of microbial liquid bacterial agent containing Pantoea camelii and its preparation, can solve the problems of easy inactivation and low content of effective bacteria, so as to promote rapid proliferation, improve nutrient absorption, The effect of extending shelf life

Active Publication Date: 2018-11-27
NANJING SHINEKING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: In order to solve the problem of low effective bacteria content and easy inactivation of existing microbial liquid inoculum, the present invention provides a microbial liquid inoculum containing Pantoea camelina, and the present invention further provides the preparation of microbial liquid inoculum method, the present invention further provides the application of microbial liquid bacterial agent

Method used

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  • Microbial liquid fungicide containing pantoea alhagi and preparation method and application of microbial liquid fungicide
  • Microbial liquid fungicide containing pantoea alhagi and preparation method and application of microbial liquid fungicide
  • Microbial liquid fungicide containing pantoea alhagi and preparation method and application of microbial liquid fungicide

Examples

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Effect test

Embodiment 1

[0046] The fermentation of embodiment 1 growth-promoting bacteria

[0047] Prepare the microbial inoculum of the present invention as follows:

[0048] Pantoea alhagi CGMCC NO.15525 stored on a slant at 4°C was inoculated on eggplant-shaped culture flask medium (ie, activation medium), and cultured at 30°C for 16 hours. Pick the strains on the activated medium, inoculate them in the fermentation medium, culture at 30°C for 40 hours, place the fermentation broth or the concentrated solution of the fermentation broth in a cold storage at 4°C for 48 hours at low temperature, and measure it by plate coating. The number of viable bacteria in the bacterial fermentation broth can reach 1×10 10 CFU / mL or more. The medium in the eggplant-shaped culture bottle is LB medium; the components of the fermentation medium are: sucrose 50g / L, beef extract 3g / L, NaCl 8g / L, peptone 8g / L, pH 6.0-8.0.

[0049] Activate the Bacillus subtilis CCTCC NO:M 2016264, Bacillus jellylike-like CCTCC NO:M2...

Embodiment 2

[0052] The preparation of embodiment 2 composite microbial bacterial agents

[0053] Prepare the compound microbial liquid bacterial agent that contains polyglutamic acid, biological organic matter, growth-promoting agent combination of the present invention according to the following method:

[0054] 1) Polyglutamic acid is prepared by fermentation (reference Xu Z, Feng X, Dan Z, et al. Enhanced poly(γ-glutamic acid) fermentation by Bacillus subtilis, NX-2 immobilized in anaerobic plant fibrous-bed bioreactor[J] .Bioresour Technol, 2014,155:8-14), the polyglutamic acid content of the fermented liquid is 15% after concentration;

[0055] 2) The molasses is purchased by the sugar factory;

[0056] 3) Mix the compound growth-promoting bacteria liquid of Example 1 with the polyglutamic acid fermentation concentrate and molasses in a ratio of 1:2.5:6.5 according to the mass ratio, and prepare the compound microbial bacterial agent of the present invention. Detect, polyglutamic a...

Embodiment 3

[0057] Embodiment 3 Composite microbial liquid inoculum can be kept open for up to 24 months

[0058] The bacterium agent used in the present embodiment is as follows:

[0059] 1) Dilute the mixed microbial agent prepared in Example 1 to an effective viable count of 7.6 × 10 9 cfu / mL, as bacterial agent 1;

[0060] 2) The mixed bacterial agent in Example 1 is mixed in proportion with the polyglutamic acid fermentation concentrate, so that the polyglutamic acid content is 4.0%, and the effective viable count is 7.6×10 9 cfu / mL, as bacterial agent 2;

[0061] 3) The mixed bacterial agent in the embodiment 1 is mixed evenly with molasses in proportion, so that the organic matter content is 63%, and the number of effective viable bacteria is 7.6×10 9 cfu / mL, as bacterial agent 3;

[0062] 4) Mix the mixed bacterial agent prepared in Example 1 with polyglutamic acid and molasses in proportion, so that the content of polyglutamic acid is 4.0%, the content of organic matter is 15%,...

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Abstract

The invention discloses a microbial liquid fungicide containing pantoea alhagi. The microbial liquid fungicide containing the pantoea alhagi comprises the following components in percentages by mass:1-10% of promoting growth bacteria fluid, 0.1-10% of polyglutamic acid, 50-70% of biological organic matters and the balance of water or aqueous solution. The promoting growth bacteria fluid comprisespantoea alhagi fluid. The invention further discloses a preparation method and application of the composite microbial liquid fungicide. The composite microbial liquid fungicide has a high-activity biological function, wherein the polyglutamic acid, the biological organic matters and a microbial agent have a synergistic effect, a microbiome environment of root systems of crops can be improved, thenumber of beneficial microorganisms in soil is increased, the quality of the crops can be improved, the yield is increased, and resistance against disadvantages such as drought resistance, salt resistance, cold resistance and disease resistance of the crops is enhanced.

Description

technical field [0001] The invention relates to a microbial inoculum, in particular to a microbial liquid inoculum containing Pantoea camelis and a preparation method and application thereof. Background technique [0002] my country is a big agricultural country in the world. With the rapid development of my country's agriculture, the use of chemical fertilizers is also increasing, reaching 1.8 million tons per year. Although this solved the problem of increasing food production and supply, it caused very serious environmental problems. The excessive use of chemical fertilizers has aggravated soil compaction and pollution, leading to the decline and degradation of soil quality, which makes the content of soil organic matter seriously lower than the global average level. Once the soil organic matter content is low, it will greatly reduce the ability to fix heavy metals, increase the harmfulness of soil heavy metals, and endanger the sustainable development of agriculture in ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 冯小海徐虹许宗奇周哲
Owner NANJING SHINEKING BIOTECH CO LTD
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