Nonreactive fermented concentrated feed for lactating sows and application thereof
A technology for sucking sows and concentrated feed, which is applied in applications, animal feed, animal feed, etc., can solve the problems of insufficient nutritional supply for suckling sows and lack of regulation of bacterial flora in sows, so as to improve production capacity and piglet survival. rate and growth rate, promote rapid recovery, reduce labor stress
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Embodiment 1
[0031] Isolation and screening of specific microorganisms: The heart of healthy weaned piglets was bled, aseptically operated, and the digestive tract was isolated. The contents of duodenum, jejunum, ileum, cecum, colon, and rectum were collected respectively, placed in 5ml sterilized normal saline and mixed for 10 minutes, and the supernatant was taken.
[0032] A. Isolation of lactic acid bacteria: spread the supernatant on MC solid medium, incubate at a constant temperature of 37°C for 24 hours, pick colonies with calcium-dissolving circles, and repeatedly streak and separate until pure strains are obtained.
[0033] B. Isolation of Bacillus: inoculate the supernatant in nutrient broth, culture at 37°C, 200r / min for 24h. Take the culture solution and place it in a water bath at 80°C for 15 minutes to kill non-spore-forming bacteria, inoculate it in nutrient broth, and culture it for enrichment at 37°C and 200 r / min for 20 hours. The bacterial solution after enrichment cult...
Embodiment 2
[0036] Preparation of bacterial strain A (lactic acid bacteria) fermentation broth:
[0037] Take 2mL strain A (viable bacteria concentration is 10 10 CFU / mL), inoculated in 100mL medium for shake flask fermentation culture, the fermentation temperature is 28-37°C, the pH value is 6.0-7.0, the rotation speed is 200-300r / min, and the fermentation time is 24-48h. Shake flask fermentation medium is MRS medium, the ingredients are: beef protein powder 10g, fish juice 10g, yeast extract juice powder 5g, glucose 20g, sodium acetate 5g, diammonium citrate 2g, magnesium sulfate 0.58g, manganese sulfate 0.28g, Add 15 g of agar powder, add 1 g of Tween 80, dissolve in 1000 mL of distilled water, and adjust the pH value to 6.0-7.0.
[0038] After the shake flask fermentation, carry out the expansion culture of the fermenter, take 100mL shake flask fermentation seed liquid and inoculate it into a 10L fermenter, the liquid volume is 5L, the fermentation temperature is 30~37℃, the pH value...
Embodiment 3
[0040] Preparation of strain B (Bacillus) fermentation broth:
[0041] Take 2mL strain B (the concentration of live bacteria is 10 10 CFU / mL), inoculated in 100mL medium for shake flask fermentation culture, the fermentation temperature is 28-37°C, the pH value is 7.2, the rotation speed is 200-300r / min, and the fermentation time is 24-36h. The shake flask fermentation medium is LB medium, the ingredients are: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH value is 7.2.
[0042] After the shake flask fermentation is completed, carry out the expansion culture of the fermenter. Take 100mL shake flask fermentation seed liquid and inoculate it into a 10L fermenter. The liquid volume is 5L. min, ferment for 24-36 hours. The composition of the medium in the pilot test of the fermenter was the same as that of the shake flask fermentation medium. After the fermentation, the medium was kept at 4°C for later use.
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