Mass Spectrometry Data Acquisition Methods

Abstract

The invention provides a data acquisition method applied to a mass spectrometer, comprising: 1. providing at least one ion source for generating ions; 2. in the first working mode of the collision cell, the ions are not or less cracked; 3. recording The mass spectrogram of the ions generated under the first working mode; 4. Select no less than one ion from the ions, and the no less than one ion is distributed in a plurality of discontinuous mass-to-charge ratio channels; 5. The collision cell In the second working mode, the selected ions are partially fragmented; 6. Record the mass spectrogram of the ion produced by the second working mode; 7. Repeat steps 2-6 several times, wherein, in the previous time The ions selected in step 4 and distributed in the discontinuous mass-to-charge ratio channels are always selected in the next repeated execution until the intensity of the selected ions is lower than a set value. The mass spectrometry data collection method of the invention can significantly improve the ion utilization efficiency and quantification ability during tandem mass spectrometry analysis.

Description

technical field [0001] The invention relates to the field of mass spectrometry data acquisition, in particular to a mass spectrometry data acquisition method which has high ion utilization efficiency and can simultaneously use the ion current diagram of daughter ions for quantitative analysis. Background technique [0002] Mass spectrometers have the characteristics of high sensitivity and good selectivity, and are widely used in the analysis of complex samples. Especially since the invention of soft ionization technology represented by electrospray ionization, mass spectrometer has been widely used in the analysis of organic matter. [0003] Common organic substances that can be qualitatively and quantitatively analyzed by mass spectrometers include proteins, peptides, metabolites, drugs, drugs, and pesticides. Since complex samples contain a large number of substances, high-resolution mass spectrometers and tandem mass spectrometers with higher resolution capabilities hav...

AI Technical Summary

Problems solved by technology

However, this method indiscriminately scans product ions for all mass-to-charge ratio windows, even in the mass-to-charge ratio windows where no precursor ions appear, which makes the scanning capacity of the mass spectrometer not fully utilized, and the ion utilization efficiency to be further improved

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