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Mass spectrometric data acquisition method

A collection method and mass spectrometry data technology, which is applied in the field of mass spectrometry data collection for quantitative analysis, can solve the problems that the ion utilization efficiency needs to be further improved, and the scanning ability of the mass spectrometer is not fully utilized, so as to achieve high ion utilization efficiency and high quantitative accuracy sexual effect

Active Publication Date: 2018-11-23
SHIMADZU SEISAKUSHO CO LTD
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Problems solved by technology

However, this method indiscriminately scans product ions for all mass-to-charge ratio windows, even in the mass-to-charge ratio windows where no precursor ions appear, which makes the scanning capacity of the mass spectrometer not fully utilized, and the ion utilization efficiency to be further improved

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Embodiment Construction

[0036] Embodiments of the present invention are described below through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation modes, and various modifications or changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that, in the case of no conflict, the following embodiments and features in the embodiments can be combined with each other.

[0037] It should be noted that the diagrams provided in the following embodiments are only schematically illustrating the basic ideas of the present invention, and only the components related to the present invention are shown in the diagrams rather than the number, shape and shape of the compo...

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Abstract

The invention provides a data acquisition method applied to a mass spectrometer, comprising the following steps: 1, providing at least one ion source for generating ions; 2, under a first working modeof a collision cell, ions being not decomposed or less decomposed; 3, recording mass spectrum of ions generated at the first working mode; 4, selecting not less than one ion from the ions, the not less than one ion being distributed in a discontinuous plurality of mass-to-charge ratio channels; 5, under a second working mode of the collision cell, the selected ions being partially cleaved; 6, recording the mass spectrum of ions generated by the second working mode; 7, repeating the steps 2-6 several times, wherein that ions selected in the discontinuous mass-to-charge ratio channel selected in the previous repetition of step 4 are selected throughout the subsequent repetition until the selected ion strength is below a set value. The mass spectrometry data acquisition method of the invention can remarkably improve the ion utilization efficiency and the quantitative ability in the cascade mass spectrometry analysis.

Description

technical field [0001] The invention relates to the field of mass spectrometry data acquisition, in particular to a mass spectrometry data acquisition method which has high ion utilization efficiency and can simultaneously use the ion current diagram of daughter ions for quantitative analysis. Background technique [0002] Mass spectrometers have the characteristics of high sensitivity and good selectivity, and are widely used in the analysis of complex samples. Especially since the invention of soft ionization technology represented by electrospray ionization, mass spectrometer has been widely used in the analysis of organic matter. [0003] Common organic substances that can be qualitatively and quantitatively analyzed by mass spectrometers include proteins, peptides, metabolites, drugs, drugs, and pesticides. Since complex samples contain a large number of substances, high-resolution mass spectrometers and tandem mass spectrometers with higher resolution capabilities hav...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01J49/02H01J49/40
CPCH01J49/02H01J49/40H01J49/0031H01J49/0045H01J49/427
Inventor 黄云清孙文剑张小强
Owner SHIMADZU SEISAKUSHO CO LTD
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