Breeding method of rapid growth strain of long mirror carps

A mirror carp, rapid technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, special data processing applications, etc., can solve the problems of poor breeding accuracy, long breeding cycle, etc., to improve production performance, speed up Breeding process, effects of high genetic diversity

Inactive Publication Date: 2018-11-23
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Claims
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Problems solved by technology

However, traditional breeding methods mainly select parents based on trait...

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  • Breeding method of rapid growth strain of long mirror carps
  • Breeding method of rapid growth strain of long mirror carps
  • Breeding method of rapid growth strain of long mirror carps

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specific Embodiment approach 1

[0011] The specific embodiment one: the breeding method of the long-shaped mirror carp fast-growing strain of the present embodiment is characterized in that the breeding method is carried out according to the following steps:

[0012] Select individuals located in different reproductive pedigrees, the genetic distance between the parents is between 0.5 and 0.7, and the offspring can enrich more than 6 dominant alleles, and carry out family or small group breeding, and the selection is completed when the offspring are sexually mature. education;

[0013] Among them, the dominant allele is selected from the 20 microsatellite marker sites in Table 1, and the target dominant genotype is also shown in Table 1:

[0014] Table 1

[0015]

[0016]

[0017] The fragment lengths and genotypes amplified from individuals without dominant alleles were inconsistent with Table 1.

specific Embodiment approach 2

[0018] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: use genetic analysis software to calculate the genetic distance between individuals and draw the evolutionary tree. Other steps and parameters are the same as those in Embodiment 1.

[0019] The genetic analysis software described in this embodiment is "molecular breeding software 1.0 based on genetic background".

specific Embodiment approach 3

[0020] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that each microsatellite-labeled forward primer is labeled with red fluorescence, green fluorescence, yellow fluorescence or blue fluorescence at the 5' end. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a breeding method of a rapid growth strain of long mirror carps. By comprehensively utilizing genetic marker information, individual quantitative trait information, genetic distance and the like, through linkage analysis, parental combination is selected, so that the production performance and the accuracy can be improved, inbreeding is avoided, and the breeding progress isshortened. The breeding method comprises the following steps that individuals which are located among different reproduction pedigrees and parental individuals and has the genetic distance between 0.5 and 0.7 are selected, family or small group combination reproduction is performed, and an offspring reaches sexual maturity, namely breeding is finished, wherein the offspring of the individuals canenrich more than 6 preponderant alleles. The method provided by the invention can maintain relatively high genetic diversity of the mirror carps, improves the production performance of the mirror carps and accelerates the breeding progress of the mirror carps.

Description

technical field [0001] The invention relates to a breeding method of mirror carps. Background technique [0002] Since the mirror carp (Cyprinus carpio L.) was introduced into my country, after decades of systematic breeding and cross-breeding, several varieties have been formed, which have the characteristics of fast growth, high quality, thick meat and suitable for processing, and have become the carp's favorite carp. One of the main breeds. However, traditional breeding methods mainly select parents based on traits, resulting in problems such as poor selection accuracy and long breeding cycle. Contents of the invention [0003] The present invention provides a genetic marker-assisted breeding method for long-shaped mirror carps. The method comprehensively utilizes genetic marker information, individual quantitative trait information, genetic distance, etc. to select and match parent combinations through linkage analysis, which can improve production performance and accu...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888G06F19/24
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 鲁翠云李超曹顶臣程磊孙效文
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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