Method for degrading sulfonylurea herbicides through composite microbial system

A technology for sulfonylureas and herbicides, which is applied in the field of degrading sulfonylurea herbicides, can solve problems such as the ecological environment of pesticide residues and the negative impact on the quality and safety of agricultural products, and achieve the effects of enriching diversity and improving degradation efficiency.

Inactive Publication Date: 2018-11-23
SHENYANG POLYTECHNIC UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The application of sulfonylurea herbicides has played an extremely important role in modern agricultural production, but the problem of pesticide residues has also caused many negative effects on the ecological environment and the quality and safety of agricultural products

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  • Method for degrading sulfonylurea herbicides through composite microbial system
  • Method for degrading sulfonylurea herbicides through composite microbial system

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] At a pH of 7.0 and a temperature of 10°C, 15°C, 20°C, 25°C and 30°C, the control group and the experimental group were placed on a constant temperature shaker, and the degradation rate was measured after 20 days of culture. The results show that 20℃ is the best temperature for the compound bacteria to degrade herbicides. At this time, the degradation rate of the four herbicides can reach more than 85%. When the temperature is 15℃, the degradation rate can reach more than 50%. Above 20°C, the degradation rate will decrease, which indicates that the composite strain is suitable for growth in a lower temperature environment.

Embodiment 2

[0046] Set the reaction temperature to 20°C and pH to 5, 6, 7, 8, 9. Place both the control group and the experimental group on a constant temperature shaker, and measure the degradation rate after 20 days of culture. The results show that the composite bacteria have the best degradation effect when pH=7. At this time, the degradation rate of the four herbicides can reach more than 85%, and when it is acid or alkaline, the degradation rate is below 30%. The composition of the compound strain is complex, the pH changes slightly, and some strains are inactivated and die due to inadaptability to the environment.

Embodiment 3

[0048] Set the reaction temperature to 20°C, pH to 7.0, and culture time to 5 days, 10 days, 15 days, 20 days and 25 days respectively. Place the control group and the experimental group on a constant temperature shaker, and measure them after 20 days of culture. Degradation rate. The results show that the degradation rate increases with the increase of time. When it is cultured for 20 days, the degradation rate can reach more than 85%, and when it is cultured for 15 days, the degradation rate can also reach 40%-50%, which shows that when the temperature is 20℃ , The pH is 7.0, and the half-life of the four herbicides is 15 days under the action of the compound strain.

[0049] Conclusion: The present invention utilizes composite bacteria to degrade sulfonylurea herbicides, and it can be known that the present invention can effectively degrade herbicides by measuring the degradation rate of the herbicides. Attaches importance to the synergy between strains, and solves the proble...

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Abstract

The invention discloses a method for degrading sulfonylurea herbicides through a composite microbial system. Four triangular flasks of 250 mL are taken, an inorganic salt culture medium of 90 mL is added into each triangular flask, and sterilization is carried out. In a sterile laboratory, a microliter of a vitamin solution and a microliter of a calcium-magnesium solution are added, a bacteria solution of 100 mL is equally divided into four parts, one part is placed in each conical flask, and constant-temperature cultivation is carried out 7 d; activation of the second time is carried out, thesame treatment is carried out in the early stage, then the whole bacteria solution is poured into a triangular flask of 500 mL, after shaken up, the bacteria solution is divided into four quarters tobe added into the four triangular flasks of 250 mL correspondingly, and constant-temperature shake cultivation is carried out 7 d; and at the third time, above cultivation is carried out, and the activated bacteria solution is obtained. Through the synergistic effect between microbial systems, the degradation efficiency on the herbicides is improved, and the diversity of sulfonylurea herbicide degradation sources is enriched; and the herbicide in-situ bioremediation mechanism is determined.

Description

Technical field [0001] The invention relates to a method for degrading sulfonylurea herbicides through a compound bacterial system. Background technique [0002] The application of sulfonylurea herbicides has played an extremely important role in modern agricultural production, but the problem of pesticide residues has also caused many negative effects on the ecological environment and the quality and safety of agricultural products. In the process of pesticide application, the actual effect of pesticides on crops only accounts for 10%-30% of the application amount. Sulfonylurea herbicides are extremely water-soluble and easily enter the soil, causing water pollution. The water cycle inhibits the surrounding and large areas. The growth of plants in the internal water body reduces the reproduction rate of aquatic organisms, destroys the ecological environment, and can cause harm to the human body through the enrichment of the food chain and affect human health. In recent years, s...

Claims

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Application Information

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IPC IPC(8): A62D3/02C12N1/20C12R1/01C12R1/38C12R1/05C12R1/025
CPCA62D3/02A62D2101/04A62D2101/28C12N1/20
Inventor 王新孙诗雨
Owner SHENYANG POLYTECHNIC UNIV
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