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Skin repair solution containing stem cell extract and preparation method thereof

A technology of skin repair and extract, which is applied in the field of skin repair, can solve problems such as tingling sensation and troubles in use, and achieve the effect of good user experience, wide sources, and obvious repair effect

Inactive Publication Date: 2018-11-23
北京原肽干细胞医学研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Stem cell skin repair solution is a common skin repair product, but although the existing stem cell skin repair solution can repair the skin, there will be a slight tingling sensation when using it, especially when it is used on the skin surface containing a wound. There is an obvious local tingling sensation, such as the patent with the notification number CN107550934 A, which brings troubles to people's use

Method used

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  • Skin repair solution containing stem cell extract and preparation method thereof
  • Skin repair solution containing stem cell extract and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A skin repair solution containing stem cell extracts, comprising the following raw materials in parts by weight: 1.6 parts of stem cell extracts, 0.6 parts of sodium hyaluronate, 15.3 parts of mannitol, 3.2 parts of nicotinamide, 2.1 parts of betaine, and oligopeptide-3 4.8 parts, 2.2 parts of polydimethylsiloxane, 0.4 parts of azone and 5 parts of Prunella vulgaris, stem cell extracts are Wharton's block fragments digested with DMEM medium containing collagenase II, filtered and used with mass fraction Subculture in DMEM medium with 8% fetal bovine serum FBS, and then replace with serum-free DMEM medium for subculture, once every 2 days, collect the cells of passage 3 for subsequent treatment; digest the cells with DMEM medium containing EDTA, and use Wash with PBS solution, resuspend in serum-free DMEM medium, lyse the broken cells by ultrasonic, centrifuge the broken product and get the supernatant; filter the supernatant with a filter with a pore size of 5000 angstro...

Embodiment 2

[0029] A skin repair solution containing stem cell extract, comprising the following raw materials in parts by weight: 2.1 parts of stem cell extract, 0.88 parts of sodium hyaluronate, 16.2 parts of mannitol, 3.64-4.4 parts of nicotinamide, 2.57 parts of betaine, oligopeptide -3 5.52 parts, polydimethylsiloxane 2.75 parts, azone 0.48 parts and Prunella vulgaris 5.63 parts, stem cell extracts are Wharton's block fragments digested with DMEM medium containing collagenase II, filtered and used containing Subculture in DMEM medium with a mass fraction of 10% fetal bovine serum FBS, and then replace with serum-free DMEM medium for subculture, once every 2 days, collect the cells of passage 5 for subsequent processing; digest the cells with DMEM medium containing EDTA , and washed with PBS solution, then resuspended in serum-free DMEM medium, ultrasonically lysed and broken cells, and the broken product was centrifuged to get the supernatant; the supernatant was filtered with a filte...

Embodiment 3

[0036] A skin repair solution containing stem cell extracts, comprising the following raw materials in parts by weight: 2.85 parts of stem cell extracts, 1.02 parts of sodium hyaluronate, 17.36 parts of mannitol, 3.98 parts of nicotinamide, 3.12 parts of betaine, and oligopeptide-3 6.76 parts, 3.04 parts of polydimethylsiloxane, 0.66 parts of azone and 6.28 parts of Prunella vulgaris, stem cell extracts are Wharton's block fragments digested with DMEM medium containing collagenase II, filtered and used with mass fraction Subculture in DMEM medium with 10% fetal bovine serum FBS, and then replace with serum-free DMEM medium for subculture, once every 2 days, collect the cells of passage 4 for subsequent treatment; digest the cells with DMEM medium containing EDTA, and use Wash with PBS solution, resuspend in serum-free DMEM medium, lyse the broken cells by ultrasonic, centrifuge the broken product and get the supernatant; filter the supernatant with a filter with a pore size of ...

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Abstract

The invention discloses a skin repair solution containing stem cell extract and a preparation method thereof. The skin repair solution is prepared from the following raw materials in parts by weight:1.6 to 3.6 parts of stem cell extract, 0.6 to 1.3 parts of sodium hyaluronate, 15.3 to 19.8 parts of mannitol, 3.2 to 4.4 parts of niacinamide, 2.1 to 3.7 parts of betaine, 4.8 to 8.5 parts of oligopeptide, 2.2 to 3.4 parts of polydimethylsiloxane, 0.4 to 0.9 part of azone, and 5 to 7.5 parts of spica prunellae. The skin repair solution has the advantages that the sources of the raw materials arewide; by adopting the active polypeptide and the stem cell extract, and compounding with other components, the multiple components can reach the synergistic function under the mutual action; the prepared finished product has obvious repair effect on the skin, any irritation feel and adverse reaction are avoided in the use process, the experience feel is good in use, and the application prospect isbroad.

Description

technical field [0001] The invention relates to the field of skin repair, in particular to a skin repair solution containing stem cell extract. Background technique [0002] The skin covers the surface of the whole body and is one of the largest organs in the human body. It has the functions of blocking the invasion of foreign bodies and pathogens, preventing the loss of body fluids, etc., and plays an important role in protecting the human body. It is thickest on the palms and soles of the feet, and thinnest on the armpits and face. The skin also has important physiological functions such as sensation, protection, and temperature regulation to maintain the opposition and unity between the body and the external environment. At the same time, the skin is an important part of the body's immune system. [0003] With the increase of age and the deterioration of the living environment, the metabolism of the skin decreases, the function of absorbing various beneficial elements de...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/73A61K8/67A61K8/44A61K8/64A61K8/9789A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/44A61K8/64A61K8/675A61K8/735A61K8/9789A61K8/99A61K2800/592A61Q19/00A61Q19/02A61Q19/08
Inventor 杨曦光刘思寒段秀芬
Owner 北京原肽干细胞医学研究院有限公司
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