Skin repair solution containing stem cell extract and preparation method thereof
A technology of skin repair and extract, which is applied in the field of skin repair, can solve problems such as tingling sensation and troubles in use, and achieve the effect of good user experience, wide sources, and obvious repair effect
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Embodiment 1
[0022] A skin repair solution containing stem cell extracts, comprising the following raw materials in parts by weight: 1.6 parts of stem cell extracts, 0.6 parts of sodium hyaluronate, 15.3 parts of mannitol, 3.2 parts of nicotinamide, 2.1 parts of betaine, and oligopeptide-3 4.8 parts, 2.2 parts of polydimethylsiloxane, 0.4 parts of azone and 5 parts of Prunella vulgaris, stem cell extracts are Wharton's block fragments digested with DMEM medium containing collagenase II, filtered and used with mass fraction Subculture in DMEM medium with 8% fetal bovine serum FBS, and then replace with serum-free DMEM medium for subculture, once every 2 days, collect the cells of passage 3 for subsequent treatment; digest the cells with DMEM medium containing EDTA, and use Wash with PBS solution, resuspend in serum-free DMEM medium, lyse the broken cells by ultrasonic, centrifuge the broken product and get the supernatant; filter the supernatant with a filter with a pore size of 5000 angstro...
Embodiment 2
[0029] A skin repair solution containing stem cell extract, comprising the following raw materials in parts by weight: 2.1 parts of stem cell extract, 0.88 parts of sodium hyaluronate, 16.2 parts of mannitol, 3.64-4.4 parts of nicotinamide, 2.57 parts of betaine, oligopeptide -3 5.52 parts, polydimethylsiloxane 2.75 parts, azone 0.48 parts and Prunella vulgaris 5.63 parts, stem cell extracts are Wharton's block fragments digested with DMEM medium containing collagenase II, filtered and used containing Subculture in DMEM medium with a mass fraction of 10% fetal bovine serum FBS, and then replace with serum-free DMEM medium for subculture, once every 2 days, collect the cells of passage 5 for subsequent processing; digest the cells with DMEM medium containing EDTA , and washed with PBS solution, then resuspended in serum-free DMEM medium, ultrasonically lysed and broken cells, and the broken product was centrifuged to get the supernatant; the supernatant was filtered with a filte...
Embodiment 3
[0036] A skin repair solution containing stem cell extracts, comprising the following raw materials in parts by weight: 2.85 parts of stem cell extracts, 1.02 parts of sodium hyaluronate, 17.36 parts of mannitol, 3.98 parts of nicotinamide, 3.12 parts of betaine, and oligopeptide-3 6.76 parts, 3.04 parts of polydimethylsiloxane, 0.66 parts of azone and 6.28 parts of Prunella vulgaris, stem cell extracts are Wharton's block fragments digested with DMEM medium containing collagenase II, filtered and used with mass fraction Subculture in DMEM medium with 10% fetal bovine serum FBS, and then replace with serum-free DMEM medium for subculture, once every 2 days, collect the cells of passage 4 for subsequent treatment; digest the cells with DMEM medium containing EDTA, and use Wash with PBS solution, resuspend in serum-free DMEM medium, lyse the broken cells by ultrasonic, centrifuge the broken product and get the supernatant; filter the supernatant with a filter with a pore size of ...
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