Method for extracting total RNA (ribose nucleic acid) of kenaf anther

An anther and kenaf technology, which is applied in the field of biochemistry, can solve the problems of easy contamination of products, difficult extraction of kenaf anther RNA, etc., and achieves the effects of improving purity and integrity, low price and simple operation.

Inactive Publication Date: 2018-11-20
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Problems solved by technology

[0003] The present invention proposes a method for extracting the total RNA of kenaf anthers, which solves the technic

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  • Method for extracting total RNA (ribose nucleic acid) of kenaf anther
  • Method for extracting total RNA (ribose nucleic acid) of kenaf anther
  • Method for extracting total RNA (ribose nucleic acid) of kenaf anther

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Embodiment 1

[0029] A kind of method of extracting kenaf anther total RNA of the present embodiment, the steps are:

[0030] 1 Preparation of RNA extraction medicines

[0031] (1) Recipe for 1 L 2% CTAB RNA Extraction Solution:

[0032]

[0033] First weigh 117 g NaCl and add appropriate amount of deionized water to dissolve, then add 50 mL 0.5mol / L EDTA-Na 2 (pH8.0) mother liquor, finally add CTAB, stir it with a glass rod to dissolve it, and then make the volume to 0.9 L, then add DEPC with a final concentration of 0.1% to make the final concentration 0.1%, shake overnight at 37°C and 200 rpm After autoclaving and autoclaving at 120 ℃, add 100mL of 1 mol / L Tris-HCl (pH8.0) mother solution and sterilize again, and add 2% (v / v) β-mercaptoethanol to the extract before use.

[0034] (2) Tris-HCl cannot be directly treated with DEPC, it needs to be prepared with 0.1% DEPC water separately.

[0035] (3) 1 mol / L Tris-HCl (pH8.0) mother solution: Weigh 121.1 Tris and put it in a 1 L beaker...

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Abstract

The invention relates to the technical field of biochemistry, in particular to a method for extracting total RNA (ribose nucleic acid) of a kenaf anther, and solves the technical problems that the kenaf anther RNA is easily degraded, and a product is easily contaminated by kenaf secondary metabolite, polysaccharide polyphenol, in an extraction process in the prior art. According to the invention,saturated guanidinium isothiocyanate is used to replace sodium chloride, and an improved CTAB method is adopted to extract the total RNA of the kenaf anther; by adopting the extraction method providedby the invention, the degradation of the total RNA of kenaf is effectively inhibited, and the purity and the integrity of the total RNA of the kenaf are improved, and the reagent cost is low, and theoperation procedure is simple.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a method for extracting kenaf anther total RNA. Background technique [0002] Kenaf is an important economic crop of bast fiber, which adapts to the low-carbon society advocated by our country, and its fertility has a great influence on the economic value of the crop. Its phloem contains nearly 75% fiber and 7% lignin, which has great utilization value in biodegradation and environmental protection. Kenaf has a variety of uses including paper, building materials, carpets, clothing and more. With the increasing global demand for fiber and the shortage of forest resources, countries all over the world are actively seeking new fiber resources, and kenaf has become an important alternative resource. With the sequencing of the kenaf genome, the research on the gene function of kenaf is in full swing; the extraction of kenaf total RNA is the most basic experiment, if the quality...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 廖小芳赵艳红何海旺周瑞阳周步进孔祥军侯文焕
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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