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Method for preparing pittosporum tobira glycoside A2

A technology of pituitary glycoside and mellow taste, applied in the chemical field, can solve the problems of no preparation methods such as pituitary glycoside A1 and pitotoxin A2, etc., and achieve the effect of high purity

Inactive Publication Date: 2018-11-20
淮安安莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no simple and mature preparation method in the prior art for the preparation of Pittoside A1 and Pittoside A2

Method used

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  • Method for preparing pittosporum tobira glycoside A2
  • Method for preparing pittosporum tobira glycoside A2
  • Method for preparing pittosporum tobira glycoside A2

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Embodiment Construction

[0049] The following describes the substantive content of the present invention in detail in conjunction with the drawings and embodiments, but does not limit the protection scope of the present invention.

[0050] 1. Experimental materials

[0051] Pittosporum leaves were collected in Zhongshan Botanical Garden, Nanjing, Jiangsu Province.

[0052] DA-201 macroporous adsorption resin was purchased from Tianjin Haoju Resin Technology Co., Ltd.

[0053] High-speed countercurrent chromatography TEB300B was purchased from Shanghai Tongtian Biotechnology Co., Ltd.

[0054] 2. Experimental methods and results

[0055] Including the following steps:

[0056] Step S1, extraction: collect Pittosporum tobira leaves, dry in the shade, pulverize, extract with 75% ethanol aqueous solution under heat reflux, solid-to-liquid ratio is 1:20, extract 3 times, each time for 1.5h, filter, combine and concentrate the filtrate until no alcohol smell;

[0057] Step S2, macroporous resin enrichm...

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Abstract

The invention discloses a method for preparing pittosporum tobira glycoside A2. The method comprises the following steps: collecting pittosporum tobira leaves, drying naturally without direct sunshine, crushing, carrying out thermal backflow extraction with a 75% ethanol solution for multiple times, filtering, combining filtrate, and concentrating till no alcohol smell; enriching the extract whichis concentrated till no alcohol smell on a DA-201 type macroporous adsorption resin column, collecting a 14-15BV eluant of 75% ethanol, concentrating till no alcohol smell, and carrying out freeze drying so as to obtain enrichment freeze-dried powder; carrying out ultrasonic dissolution on the enrichment freeze-dried powder with an upper phase and a lower phase of an equal volume, carrying out HSCCC (High-Speed Counter Current Chromatography) separation purification, collecting an eluant component corresponding to pittosporum tobira glycoside A2according to a spectrum, and concentrating and drying, thereby obtaining pittosporum tobira glycoside A2 respectively, wherein the HSCCC adopts a two-phase solvent system consisting of n-butyl alcohol / ethyl acetate / isopropanol and water in a volumeratio of 2:16:3:16. The preparation method disclosed by the invention is simple and easy to industrialize, and the prepared pittosporum tobira glycoside A2 is high in purity.

Description

technical field [0001] The invention belongs to the field of chemistry, and relates to a preparation method of pitoside A1 and pitoside A2. Background technique [0002] Pittosporin A1 and Pitotonia glycoside A2 are natural products present in the leaves of Pitotonia chinensis. The chemical structure is as follows: [0003] [0004] Studies have found that Pittoside A1 and Pittoside A2 have excellent anti-tumor activity (Chinese patents 201811039559X, 2018110400206, 2018110400210), and have the prospect of being developed into anti-tumor drugs. [0005] At present, there is no simple and mature preparation method in the prior art for the preparation of Pittoside A1 and Pittoside A2. Contents of the invention [0006] The purpose of the present invention is to overcome the deficiencies of the prior art, and provide a preparation method of Pittoside A1 and Pitotoside A2. [0007] The above object of the present invention is achieved through the following technical solut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/24C07H1/08
CPCC07H15/24C07H1/08
Inventor 郑嘉荣万超吕学金
Owner 淮安安莱生物科技有限公司
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