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Breeding method of high-yielding Jersey cows

A technology for dairy cows and Jersey, which is applied in the field of high-yield milk-producing Jersey dairy cow breeding, can solve problems such as unreported performance, and achieve the effect of improving breeding efficiency.

Active Publication Date: 2022-03-08
GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report on the milk production performance of Jersey cows

Method used

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  • Breeding method of high-yielding Jersey cows
  • Breeding method of high-yielding Jersey cows
  • Breeding method of high-yielding Jersey cows

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1 Materials and methods

[0026] 1.1 Test materials

[0027] 20 high-yielding (305 d milk yield > 4000 kg) and 20 low-yielding (305 d milk yield < 3200 kg) Jersey cattle were provided by Guangxi Zhuang Autonomous Region Animal Husbandry Research, jugular vein blood 10 mL, EDTA-k2 anticoagulant, - Store at 20°C.

[0028] Primers and main reagents: 250 S series random primers; Taq DNA polymerase, restriction endonuclease EcoRI, DNA Marker DL2 000, dNTP and DNA recovery kit were all provided by Japan TaKaRa Company.

[0029] 1.2 Test method

[0030] 1.2.1 DNA extraction

[0031] The total DNA of dairy cow blood was extracted with a DNA extraction kit for electrophoresis detection, and the DNA concentration of individual dairy cows was detected with a UV spectrophotometer, and diluted to the same concentration for later use. Take the same amount of DNA from each individual and mix them into high-yield cow DNA pool and low-yield cow DNA pool.

[0032] 1.2.2 Optimization...

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Abstract

The invention belongs to the technical field of bioengineering, in particular to a breeding method for high-yielding Jersey cows. In this application, 250 10-base random primers were used to perform RAPD-PCR between two DNA pools composed of 20 high-yield (305d milk yield>4000kg) and 20 low-yield (305d milk yield<3200kg) Jersey cattle. Amplified molecular marker screening method. All individuals were tested by PCR with 200 primers screened, and three RAPD markers related to milk production traits of dairy cows were obtained, and they were cloned, sequenced and analyzed by bioinformatics. The test results showed that the DNA fragments of S11, S1110 and S1120 were related to milk yield, among which S11 was positively correlated with milk yield traits, and S1110 and S1120 were negatively correlated with milk yield traits.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a breeding method for high-yielding Jersey cows. Background technique [0002] Jersey cattle are imported dairy cows from Jessie Island in the English Channel. They are precious cattle breeds protected by laws issued by the British government. Their biggest advantage is that they have thick milk, and their milk fat and protein content are significantly higher than ordinary cows. The milk fat percentage is average. It is 5.5-6%, and it is a famous breed of dairy cows. The breed is genetically related to the Brown Swiss, Devin and Kerry cattle, but not to the Holstein cattle. During the early Jersey breeding of the breed, it was known as Alderney. Jerseys are one of the smallest of the major dairy breeds (Kerrys and Dexters are smaller). Compared with other breeds, this breed has strong heat resistance and is famous for its good feed intake and high milk fat and milk prot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 黄明光梁金逢周晓情肖正中吴柱月朱文方治山文信旺彭夏云潘锐
Owner GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
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