Typing detection method for applying loop-mediated isothermal nucleic acid amplification technology to alveolar echinococcus/echinococcus granulosus and detection kit

Abstract

The invention relates to a typing detection method for applying a loop-mediated isothermal nucleic acid amplification technology to alveolar echinococcus / echinococcus granulosus and a detection kit. The typing detection method comprises designing an LAMP primer for distinguishing alveolar echinococcus / echinococcus granulosus, selecting LAMP amplification conditions and detecting an LAMP amplification product. The method can perform typing detection on a sample containing alveolar echinococcus / echinococcus granulosus, is high in accuracy, is high in sensitivity, and is simple and convenient tooperate; and amplification results can be directly observed through naked eyes.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for applying a loop-mediated isothermal nucleic acid amplification technique (LAMP) to the typing detection of multilocular / granulococcus granulosus. Background technique [0002] Loop-mediated isothermal nucleic acid amplification technology, that is, LAMP (Loop-mediated isothermal amplification) is a new technology that does not require deformation annealing extension under isothermal conditions, and only needs the simplest water bath to perform nucleic acid amplification. Primer design Mainly for six different regions of the target gene, 4 kinds of primers were designed based on 6 different sites such as F3c, F2c and Flc regions at the 3' end of the target gene and B1, B2 and B3 regions at the 5' end, including FIP (Forward InnerPrimer): Upstream internal primer, consisting of F2 region and F1C region, F2 region is complementary to the F2c reg...

AI Technical Summary

Problems solved by technology

However, due to the small size of echinococcosis, there is no obvious difference in the observed morphology of routine microscopic examination, and the modification method is time-consuming and labor-intensive with low sensitivity. At present, real-time quantitative PCR typing of MGB probes has been used to detect echinococcosis multilocularis and acanthus granulosus. The method of coccidia, but requires special detection equipment (fluorescent quantitative PCR instrument), not suitable for use at the grassroots level

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