A kind of dragon fruit tissue culture method of inducing adventitious buds with leaves

A technology of tissue culture and dragon fruit, which is applied in the field of plant tissue culture, can solve the problems commonly seen in dragon fruit seeds, stems and other parts, and achieve the effects of short culture period, shortened seedling cultivation process, and convenient materials

Inactive Publication Date: 2019-11-22
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are studies related to tissue culture of dragon fruit in foreign countries, the explants used are mostly found in the seeds, stems and other parts of dragon fruit. There is no report on the direct induction of adventitious buds by using dragon fruit leaves as explants

Method used

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  • A kind of dragon fruit tissue culture method of inducing adventitious buds with leaves
  • A kind of dragon fruit tissue culture method of inducing adventitious buds with leaves
  • A kind of dragon fruit tissue culture method of inducing adventitious buds with leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of explant

[0034] The test material Longzhuguo is a seedling that grows for three months and is about 12cm high, which is cultivated from seed sowing. Choose young, strong, and basically the same growth of dragon pearl fruit leaves as explants, rinse them under running water, and then rinse them with sterile water 3 times. Put the cleaned leaves on the ultra-clean workbench, first wipe the front and back of the leaves with a cotton ball dipped in 75% ethanol aqueous solution, then wash with sterile water 3 times, and then use the final concentration as the quality Tween-20 with a fraction of 0.02% and a mass fraction of 0.1% HgCl 2 The solution is soaked for 3 minutes, and finally rinsed with sterile water 5 times. Cut the sterilized leaves into small squares about 1cm×1cm as explants.

[0035] (2) Inducing adventitious buds

[0036] Put the explants into the adventitious bud induction medium with the leaf adaxial side facing up. Adventitious bud inductio...

Embodiment 2

[0042] (1) Preparation of explant

[0043] The test material Longzhuguo is a seedling that grows for three months and is about 8cm high, which is cultivated from seed sowing. Select young, strong, and basically the same growth of dragon pearl fruit leaves as explants, rinse them under running water, and then wash them with sterile water twice. Place the cleaned leaves on a clean workbench, first wipe the front and back sides of the leaves with cotton balls dipped in 75% ethanol aqueous solution, and then wash them twice with sterile water, and then use the final concentration as the quality Tween-20 with a fraction of 0.02% and a mass fraction of 0.1% HgCl 2 Soak the solution for 5 minutes, and finally rinse with sterile water 4 times. Cut the sterilized leaves into small squares about 1cm×1cm as explants.

[0044] (2) Inducing adventitious buds

[0045] Put the explants into the adventitious bud induction medium with the leaf adaxial side facing up. Adventitious bud induction me...

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Abstract

The invention discloses a dragon fruit tissue culture method using blades to induce adventitious buds. The dragon fruit blades are used as explants and are disinfected to obtain explants with extremely low pollution rate and less damage, the successfully-disinfected explants induce adventitious buds in an adventitious bud induction medium, and then dragon fruit plants growing well are obtained through rooting and proliferation, transplanting and other processes. The dragon fruit tissue culture method has the characteristics of short culture period, high speed and quick emergence, has the advantages of high material availability, high material utilization rate, short breeding period and simple operation, is suitable for factory production, establishes a tissue culture rapid propagation system with dragon fruit blades as explants, and provides seedling technology for rapid propagation of the dragon fruit.

Description

Technical field [0001] The invention belongs to the field of plant tissue culture, and specifically relates to a dragon pearl fruit tissue culture method using leaves to induce adventitious buds. Background technique [0002] Dragon pearl fruit (Passiflorafoetida L.), also known as fragrant flower fruit, celestial fruit, wild peach, etc., is a herbaceous liana of the Passiflora family. It is native to the West Indies and is mainly distributed in Guangdong, Hainan, Fujian and Taiwan in my country. It grows on barren hills, grass slopes, shrubs or beaches near the sea (Wang Dong et al., 2014). [0003] The dragon pearl fruit plant is several meters high, and the whole plant, especially the damaged leaves, often exudes a pungent taste; the stems are striped and have flat pilose; the leaves are membranous with pilose on the surface, broadly oval or oblong ovoid, and the base is centered. Shaped and with 3 to 5 lobed, irregular edges, pinnate veins; petioles with spreading pilose; stip...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 段俊刘艳艳何春梅
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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