Immunodeficient mouse model constructed by utilizing NK/T lymphoma cell strain

Abstract

The application belongs to the technical field of construction of animal tumor models, and particularly relates to a stably passaged immunodeficient mouse model constructed by utilizing an NK / T lymphoma cell strain and a construction method of the model. The method comprises the following steps: pretreating an innoculation object and a lymphoma cell strain respectively, preparing tumor suspensionfor injecting into a mouse, enabling tumor cells to passage and the like. The immunodeficient mouse model can be used for drug screening or therapeutic effect evaluation in medical science. The construction method for constructing the lymphoma animal model by utilizing the NK / T lymphoma cell strain, which is provided by the application, utilizes the fixing effect of matrigel, can be used for fixing lymphoma cells beneath skin, so that the tumor formation rate of the mouse is ensured, and the technical advantages of fast tumor formation and high tumor formation rate are manifested; on the otherhand, in the process of passage, the method cooperates with the cryopreserved operation of a relevant tumor tissue culture solution, and the lymphoma animal model for passage can be recovered at anymoment and directly constructed.

Description

technical field [0001] This application belongs to the technical field of animal tumor model construction, and specifically relates to a patent application for an immunodeficiency mouse model constructed by using NK / T lymphoma cell lines that can be stably passaged and its construction method. Background technique [0002] The construction of animal tumor models is one of the commonly used research specimen materials in related diseases, especially in tumor research. Among them, the tumor xenograft model is one of the main targets for the construction of animal tumor models because of its great application value in the screening of new anti-tumor drugs and the evaluation of curative effects. [0003] Immunodeficient animals are implanted with established cell lines or patient tumor tissues to construct tumor xenograft models, which is a commonly used method for constructing tumor models. However, it is relatively easy to use this construction method to construct animal mode...

AI Technical Summary

Problems solved by technology

However, it is relatively easy to use this construction method to construct animal models of solid xenograft tumors, but for the construction of lymphoma animal models, especially NK / T lymphoma animal models, there are obvious technical defects that cannot be stably passaged

Therefore, there have been no good reports on the construction of immunodeficiency mouse models that can be stably passaged using specific lymphoma cell lines.

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