Helicobacter pylori immunodominant epitope peptide L79-96 and application of same
A Helicobacter pylori, immunodominant technology, applied in the field of bioengineering
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Embodiment 1
[0025] 1 Methods and Materials
[0026] 1.1 Patients and blood samples
[0027] Collection of samples was performed according to a protocol approved by the Human Ethics Review Board of Nanfang Hospital Affiliated to Southern Medical University, Guangzhou, and all participants gave written informed consent. The status of H.pylori infection in 30 gastric disease patients was confirmed by endoscopy and pathological diagnosis. Blood samples were collected, and peripheral blood mononuclear cells (PBMCs) were isolated using the Ficoll-Hypaque (TBDscience, Tianjin, China) density gradient method and then stored in liquid nitrogen. The HLA typing of PBMCs from H. pylori patients was identified by PCR-SBT, which was identified by Huada Genomics Research Institute (Shenzhen, China).
[0028] 1.2 Recombinant antigens and synthetic peptides
[0029] The expression and purification of recombinant Lpp20 (rLpp20) of H. pylori NCTC11639 strain in E. coli refer to the article [Li Y, Ning YS...
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