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Method of preparing multiple products from fresh tobacco leaves

A technology for fresh tobacco leaves and products, applied in the field of bioengineering, can solve the problems of not reflecting the principle of comprehensive utilization of fresh tobacco leaves, not taking into account the full utilization, low utilization rate of raw materials, etc., achieving rich and balanced amino acid composition, improving comprehensive utilization rate, The effect of high utilization of raw materials

Active Publication Date: 2018-11-06
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the extraction and separation of effective substances from fresh tobacco leaves, and the published patents mainly focus on the extraction, separation and preparation of single products such as solanesol and organic fertilizer. The utilization rate of raw materials is low, and the principle of comprehensive utilization of fresh tobacco leaves is not reflected

Method used

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  • Method of preparing multiple products from fresh tobacco leaves
  • Method of preparing multiple products from fresh tobacco leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the mensuration of each active ingredient content in the fresh tobacco leaf

[0033] Using crushed fresh tobacco leaves as raw materials, the contents of chlorophyll, nicotine, solanesol, spices and protein were determined by different methods.

[0034] Determination of each active ingredient content in the fresh tobacco leaf of table 1

[0035]

Embodiment 2

[0036] The pretreatment of embodiment 2 tobacco leaves

[0037] Fresh tobacco leaves collected in Yunnan Province were first crushed with a traditional Chinese medicine grinder at 25,000r / min for 30s, followed by suction filtration and centrifugation for solid-liquid separation to obtain solid tobacco leaves and eluate. 1000g of fresh tobacco leaves were pretreated 336g solid tobacco leaves and 627g eluate were obtained, and the yields of solid tobacco leaves and eluate were 33.6% and 62.7% respectively.

Embodiment 3

[0038] Embodiment 3: the extraction of chlorophyll (1)

[0039] Carry out the extraction of chlorophyll with the 336g solid tobacco leaf that embodiment 2 obtains as raw material, add 90% (v%) ethanol solution according to solid-liquid ratio is 1:15 (g:g), heat reflux extraction at 50 DEG C for 30min, extraction number of times For 1 time, 5031mL chlorophyll extract solution I and 330g soot residue were obtained after suction filtration, the extraction rate of chlorophyll was 45.3%, and the yield of soot residue was 98.21%.

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Abstract

The invention provides a method of preparing multiple products from fresh tobacco leaves. According to the method, six products (sodium copper chlorophyll, nicotine, solanesol, spice, proteins, and abacterial fertilizer) are prepared from fresh tobacco leaves; and the comprehensive utilization rate of fresh tobacco leaves is improved. Compared with the prior art, the chlorophyll extraction efficiency is further improved, the yield and purity of prepared sodium copper chlorophyll are both increased, and prepared sodium copper chlorophyll does not contain any impurity such as nicotine. In addition to nicotine, the contents of solanesol and spice in the extract are also increased, and the protein extraction rate is high. The microbial fertilizer, which is prepared by fermenting tobacco dregs, has the advantages of short period and high yield of spores. The provided method has the advantages of low cost, simple technology, high efficiency, rapidness, and easiness for industrial amplification, is economic and environmentally-friendly, and is capable of comprehensively utilizing the effective components of tobacco leaves.

Description

technical field [0001] The invention relates to the field of bioengineering, and can simultaneously prepare six products of copper chlorophyllin sodium salt, nicotine, solanesol, spices, protein and bacterial fertilizer, and specifically relates to a coproduction method for preparing the six products from fresh tobacco leaves. Background technique [0002] my country's tobacco planting area and output rank first in the world, and nearly one million tons of discarded fresh tobacco leaves are produced in tobacco fields every year. Fresh tobacco leaves are rich in chlorophyll, nicotine, solanesol, spices, proteins and other natural products, among which chlorophyll and its derivatives have significant effects in antibacterial, antimutagenic and deodorizing. Nicotine has good application prospects in the preparation of insecticides and the prevention of Alzheimer's disease. Solanesol has antibacterial, anti-inflammatory and hemostatic effects. It is an important pharmaceutical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/22C07D401/04C07C29/74C07C33/02C07K1/30C05F5/00C05F17/00C11B9/02
CPCC05F5/006C07C29/74C07D401/04C07D487/22C07K1/30C11B9/025C05F17/20C07C33/02C05F11/08Y02W30/40Y02A40/20
Inventor 修志龙栾倩倩董悦生戴建英
Owner DALIAN UNIV OF TECH
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