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Method for tissue culture and rapid propagation seedling growth of holboellio latifolia wall axillary buds

A technology for rapid seedling breeding and axillary buds, applied in the field of seedling breeding, can solve the problems of low reproduction coefficient, inconsistent fruit maturity and size, poor rooting rate, etc., and achieve the effect of high reproduction coefficient, developed root system, and improved success rate.

Inactive Publication Date: 2018-11-06
湖北襄草源生态农业科技有限公司
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Problems solved by technology

[0010] The object of the present invention is to provide a kind of August melon axillary bud tissue culture rapid propagation seedling method, to solve the problem of low reproduction coefficient of propagation mode, poor rooting rate, fruit maturity and size inconsistency

Method used

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Embodiment Construction

[0014] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0015] 1. Operating equipment: scissors, tweezers, sterile petri dish, test tube medium, differentiation medium, rooting and strong seedling medium.

[0016] 2. Operation process:

[0017] 1. Collection and disinfection of August melon axillary buds

[0018] Before germination in early spring, pick the axillary buds of large August melon trees with good quality and no pests and diseases. Take the axillary buds of August melon with a length of 5-10 cm. After removing the young leaves of the axillary buds of August melon, rinse them ...

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Abstract

The invention relates to a method for tissue culture and rapid propagation seedling growth of holboellio latifolia wall axillary buds. The method comprises (1) holboellio latifolia wall axillary bud collection and disinfection, (2) inoculation, (3) differentiation culture, (4) rooting and seedling strengthening culture and (5) seeding hardening and transplanting. The holboellio latifolia wall axillary buds are cleaned and disinfected before holboellio latifolia wall inoculation so that the success rate of the holboellio latifolia wall inoculation is improved. Through control of the temperatureand light intensity, the holboellio latifolia wall axillary buds can fast breed in a short time period and be subjected to differentiation culture, the holboellio latifolia wall seedlings with differentiated stems and leaves are transplanted to the rooting and strong seedling medium, and under suitable conditions, a bottle of the holboellio latifolia wall mother seedlings breed into tens of thousands of test tube rooting seedlings in one year. The holboellio latifolia wall seedlings obtained by the method has a high propagation coefficient, strong bodies and developed root systems. The methodimproves a seedling rate and ensures that the fruit maturity and size are not much different.

Description

technical field [0001] The invention relates to the technical field of seedling cultivation, in particular to tissue culture and rapid propagation of seedlings of axillary buds, in particular to a method for tissue culture and rapid propagation of August melon axillary buds. Background technique [0002] August melon is the fruit of Akebia genus Akebia trilobata in the family Akebia. It is also known as cow cashew fruit, dog kidney, August fried, September fried, etc. It is named after the fruit in August is ripe and cracked. , vitamin C and 12 kinds of amino acids, as well as valine acid, methionine, isoleucine, phenylalanine, lysine, etc. that cannot be synthesized by the human body. Fruity and sweet, it is a pollution-free green food. It is called "soil banana". It is distributed in Guizhou and Hunan. In August, melons are ripe, and there are abundant wild resources in Suoxiyu, Yangjiajie and other foothill valleys and forest edge bushes. [0003] August melon is an eve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈风雷
Owner 湖北襄草源生态农业科技有限公司
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