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Seedling method for celosia cristata

A technology of cockscomb and tissue culture seedlings, applied in the field of plant propagation, can solve the problems of low efficiency and low survival rate, and achieve the effects of cost reduction, large number of roots, and enhanced secretion

Inactive Publication Date: 2018-11-06
金华市野泉园艺技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But only by seed propagation, its survival rate is not high, and the efficiency is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The seedling raising method of celosia, specifically comprises the following steps:

[0019] 1) Pick cockscomb seeds that are plump in shape and nearly intact in size, sterilized with 0.5% sodium hypochlorite solution for 10 minutes, rinsed with distilled water 4 times, put into an ice bath at 0°C for 3 hours to germinate, and the water contains 5g / L ethanol, 12mg / L For LN-benzylquinine chloride, dry the water with absorbent paper, put a layer of filter paper and double-layer gauze in the culture dish, put the seeds in the incubator for germination; the temperature of the incubator is 24°C, and the light time is 12h, the light intensity is 2500Lux, spray water every day, and stop spraying after germination; 2) Cut the terminal buds of sterile seedlings and insert them into MS medium, sprinkle 1mm thick activated carbon powder into the medium, control the induction temperature to 23°C, and light The time is 12 hours, the light intensity is 2600Lux, and the continuous cul...

Embodiment 2

[0021] The seedling raising method of celosia, specifically comprises the following steps:

[0022] Germination: Pick cockscomb seeds that are plump in shape and nearly intact in size, disinfect them with 1% sodium hypochlorite solution for 20 minutes, wash them with distilled water for 5 times, put them in an ice bath at 1°C for 5 hours, and the water contains 8g / L ethanol, 15mg / L For LN-benzylquinine chloride, dry the water with absorbent paper, put a layer of filter paper and double gauze in the culture dish, put the seeds in the incubator for germination; the temperature of the incubator is 26 °C, and the light time is 14h, the light intensity is 2600Lux, spray water every day, and stop spraying after germination; adding a small amount of N-benzylquinine chloride and an appropriate amount of ethanol in the germination-inducing aqueous solution can help in accelerating germination. Under the action of an appropriate amount of ethanol, N- Benzyl quinine chloride can stimulat...

Embodiment 3

[0027] The seedling raising method of cockscomb comprises: germination acceleration, first-generation bud induction, subculture multiplication culture, and tissue culture seedling rooting, specifically comprising the following steps:

[0028] Germination: Pick cockscomb seeds that are plump in shape and nearly intact in size, disinfect them with 0.8% sodium hypochlorite solution for 15 minutes, wash them with distilled water 4 times, put them in an ice bath at 1°C for 4 hours, and the water contains 6g / L ethanol, 12mg / L For LN-benzylquinine chloride, dry the water with absorbent paper, put a layer of filter paper and double gauze in the culture dish, put the seeds in the incubator for germination; the temperature of the incubator is 25°C, and the light time is 12h, the light intensity is 2500Lux, spray water every day, stop spraying after germination; adding a small amount of N-benzylquinine chloride and an appropriate amount of ethanol in the germination promoting water soluti...

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PUM

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Abstract

The invention discloses a seedling method for celosia cristata. The seedling method comprises the steps of germination acceleration, primary bud induction, subculture multiplication and tissue cultureseedling rooting. The seedling method comprises the following specific steps: firstly, taking celosia cristata seeds, sterilizing and soaking the seeds into an aqueous solution containing ethanol andN-benzyl quinine chloride; secondly, culturing sprouts, taking terminal buds of the sprouted aseptic seedlings, and inoculating the terminal buds into an MS culture medium for the primary bud induction; then taking 2 to 3 sections of bud segments and inoculating the bud segments into the MS culture medium for carrying out subculture multiplication; finally, taking seedlings, transferring the seedlings into a rooting culture medium for carrying out tissue culture rooting, carrying out seedling hardening and then transplanting. The seedling method disclosed by the invention has the advantages that a large amount of multiplied seedlings can be obtained, the cost is greatly reduced, and breeding of the celosia cristata is accelerated; by use of the germination acceleration step, the proteinase secretion rate and the respiration rate of the celosia cristata seeds can be increased, the glycolytic cycle and the mitochondria activity are further enhanced, and seed awakening is accelerated; the celosia cristata seedlings have developed root systems, great root number, thick diameter and high survival rate.

Description

technical field [0001] The invention relates to the field of plant propagation, in particular to a method for raising seedlings of cockscomb. technical background [0002] Celosia (scientific name: Celosia cristata L. ), Chinese aliases: Jijihua, Laolaihong, Luhua cockscomb, pen cockscomb, small-headed cockscomb, phoenix-tailed cockscomb, big cockscomb flower, chicken horn root, red cockscomb. It is a one-year herbaceous plant that blooms in summer and autumn. The flowers are mostly red and cockscomb in shape, so it is called. Native to Africa, tropical America and India. Hi sunny, hot and humid, intolerance to frost. Not resistant to barrenness, likes loose, fertile and well-drained soil. Widely cultivated all over the world, common garden plant. In addition, it has high medicinal value. The propagation of cockscomb mostly adopts the seed propagation method. During the Qingming period, select a good plot, apply enough base fertilizer, plow finely and rake evenly, leve...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01C1/08A01H4/00
CPCA01C1/00A01C1/08A01H4/001A01H4/008
Inventor 王志刚
Owner 金华市野泉园艺技术有限公司
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