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A method for improving the yield of triterpenes produced by fermentation of Inonotus obliquus

A technology of Inonotus obliquus and yield, applied in the field of fermentation engineering, can solve the problems of low medicinal value, low utilization rate of Eucommia samara shell, waste of resources, etc., and achieve low cost, low utilization rate, The effect of short production cycles

Active Publication Date: 2021-05-14
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the use of Eucommia to increase the production of triterpenoids in Inonotus obliquus. As we all know, Eucommia is a unique medicinal plant in my country with rich resources. Eucommia bark is a traditional Chinese medicine with high medicinal value. The medicinal value is not high, and the technology of extracting natural rubber from Eucommia samara shell is still immature, resulting in extremely low utilization rate of Eucommia samara shell, resulting in a waste of resources

Method used

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  • A method for improving the yield of triterpenes produced by fermentation of Inonotus obliquus
  • A method for improving the yield of triterpenes produced by fermentation of Inonotus obliquus
  • A method for improving the yield of triterpenes produced by fermentation of Inonotus obliquus

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Embodiment 1

[0030] Liquid fermentation medium: corn flour 53g / L, peptone 3g / L, CoCl 2 0.02g / L, KH 2 PO 4 1g / L,MgSO 4 0.2g / L, ZnSO 4 0.01g / L, K 2 HPO 4 0.5g / L,FeSO 4 0.05g / L, CuSO 4 0.02g / L, MnSO 4 0.09g / L, Eucommia bark powder 40g / L, natural pH.

[0031] 10% inoculum of Inonotus obliquus was cultured on a constant temperature shaker at 28°C and 136 rpm.

[0032] The dynamic change of Eucommia bark on the mycelium biomass of Inonotus obliquus: The total amount of Inonotus obliquus mycelium was measured by sampling every 24 hours. Adding Eucommia bark medium, the biomass of Inonotus obliquus mycelium reached the maximum value of 7.1g / L on the eighth day, and the effect on the maximum biomass was not significant ( figure 1 ).

[0033] Dynamic changes of Eucommia bark on triterpenoids produced by Inonotus obliquus: samples were taken every 24 hours to determine the total triterpenoid content in Inonotus obliquus mycelium. Adding Eucommia bark medium, the total triterpenoid p...

Embodiment 2

[0036] Liquid fermentation medium: corn flour 53g / L, peptone 3g / L, CoCl 2 0.02g / L, KH 2 PO 4 1g / L,MgSO 4 0.2g / L, ZnSO 4 0.01g / L, K 2 HPO 4 0.5g / L,FeSO 4 0.05g / L, CuSO 4 0.02g / L, MnSO 4 0.09g / L, Eucommia samara shell powder 40g / L, natural pH.

[0037] 10% inoculum of Inonotus obliquus was cultured on a constant temperature shaker at 28°C and 136 rpm.

[0038] The dynamic change of Eucommia samara shell on the mycelium of Inonotus obliquus: Sampling every 24 hours to determine the total amount of Inonotus obliquus mycelium. Add in Eucommia samara shell culture medium, the Inonotus obliquus mycelium biomass reaches maximum value on the fourth day and is 14.5g / L, improves 101.4% than matched group (liquid fermentation basal medium) ( image 3 ).

[0039] The dynamic change of Eucommia samara shell on the production of triterpenoids by Inonotus obliquus: Samples were taken every 24 hours to determine the total triterpenoid content in Inonotus obliquus mycelium. Add ...

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Abstract

The invention discloses a method for increasing the yield of triterpenes produced by fermentation of Inonotus obliquus. Inonotus obliquus is used as a fermenting bacterium, and a fermentation medium is formed by adding a triterpene yield promoter to a liquid fermentation basic medium. The yield of triterpenes is The additive amount of the accelerator is 10-60g / L, and it is submerged in liquid to increase the production of triterpenes. The invention has low equipment investment, simple post-treatment, basically no pollution, low cost and high resource utilization rate, and can significantly increase the yield of triterpenoids in liquid submerged fermentation of Inonotus obliquus.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a method for increasing the yield of triterpenes produced by fermentation of Inonotus obliquus. Background technique [0002] Inonotus obliquus, also known as chaga, is a medicinal fungus that grows on birch trees. It has a variety of medicinal components, strong biological activity and a wide range of pharmacological effects. The Moscow Academy of Medical Sciences once identified Inonotus obliquus as an anticancer substance; the United States classified it as a "special natural substance"; Japan used it as a therapeutic agent to treat AIDS, liver cancer and 0-157 Escherichia coli poisoning. The Ainu people in northern Japan drink hot water extracts of Inonotus obliquus sclerotia to treat stomach pains and some inflammations; in northern and central Russia, Inonotus obliquus extracts are often used to lower arterial and venous blood pressure in patients, for In ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02A61K36/07A61P35/00C12R1/645
CPCA61K36/07A61K2236/19A61P35/00C12P1/02
Inventor 徐向群金黎达
Owner ZHEJIANG SCI-TECH UNIV
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