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Preparation containing bacillus subtilis YL13 for antagonizing diseases and/or promoting growth of plants and application of the preparation

A technology of Bacillus subtilis and diseases, applied in the direction of plant growth regulators, plant growth regulators, applications, etc., can solve the problems of few broad-spectrum biocontrol strains, limit the scope of use of strains, and have no significant inhibitory effect, etc., to achieve The effect of low cost, outstanding effect and simple preparation method

Active Publication Date: 2018-10-23
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the relevant technology is still very weak at present, and the strains screened have a single function
For example: the application number is that 200910042461.4 discloses a kind of Bacillus subtilis Y13 of efficient prevention and treatment of camellia oleifera anthracnose, but the control effect of this bacterial strain still needs to be further improved, and this bacterial strain only has certain control effect to camellia oleifera anthracnose, to Other types of anthracnose, such as Dalbergia japonica, sandalwood anthracnose bacteria are not significantly inhibited, which greatly limits the scope of use of this strain
Most of the reported biocontrol strains can only be effective against individual species or a few types of bacteria, and few broad-spectrum biocontrol strains appear

Method used

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  • Preparation containing bacillus subtilis YL13 for antagonizing diseases and/or promoting growth of plants and application of the preparation
  • Preparation containing bacillus subtilis YL13 for antagonizing diseases and/or promoting growth of plants and application of the preparation
  • Preparation containing bacillus subtilis YL13 for antagonizing diseases and/or promoting growth of plants and application of the preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, the obtaining process of bacterial strain YL13 of the present invention

[0045] Using ultraviolet rays to mutate the Bacillus subtilis Y13 with the preservation number CCTCC NO:M 208264, using Dalbergia anthracnose as the pathogenic bacteria, the best mutagenic strain Uv-8 was obtained by screening, and the bacteriostatic rate was above 80.75%; -Nitrosoguanidine compound mutagenesis, screened and obtained the best mutant strain NTG1-8, the bacteriostatic rate reached 86.47%, which was 12.79% higher than the original strain Y13, and it was renamed as YL13.

[0046]

[0047]

[0048] Bacteriostatic rate is control effect

[0049] Table 1 Ultraviolet Mutation Shake Flask Re-screening Results

[0050]

[0051] Table 2 Compound mutagenesis re-screening results

[0052]

Embodiment 2

[0053] Embodiment 2: the bacteriostasis comparison of mutagenic strain YL13 and starting strain Y13

[0054] Table 3 Inhibitory effect of mutagenic strain Y13 on plant pathogenic fungi

[0055]

[0056] Inhibitory effect of table 4 mutagenic strain YL13 on phytopathogenic fungi

[0057]

[0058]

Embodiment 3

[0059] Embodiment 3: the preparation of biocontrol agent of the present invention

[0060] The carriers and auxiliaries in Table 5 were specifically tested for their influence on the growth of YL13 by plate counting method.

[0061] Table 5 Effects of different carriers and additives on the growth of YL13

[0062]

[0063] Original drug: YL13 fermentation broth

[0064] (1) Preparation of the initial dosage form: the optimal carrier bentonite and the original drug (thalline concentration 8.0×10 10 cfu / mL) according to the mass ratio of material to liquid of 7:1, mixed evenly, dried in an oven at 50±1°C, and crushed into powder.

[0065] (2) Screening of wetting agents: wettability is one of the important indicators to measure the quality of water-dispersible granules, because sodium butyl naphthalene sulfonate has a slightly stronger inhibition on YL13 than tea dry powder, but its wetting The time is shorter, so sodium butyl naphthalene sulfonate is preferred. Using the...

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Abstract

The invention discloses a preparation containing bacillus subtilis YL13 for antagonizing diseases and / or promoting growth of plants and an application of the preparation. The strain, bacillus subtilisYL13, is assigned the accession number CCTCC No. M2018008 and is a broad-spectral antagonistic strain that can antagonize the plant diseases and is obtained through mutagenesis for the first time. The strain has significant effect of inhibition of colletotrichum camelliae, fusarium proliferatum, agaricodochium camelliae, colletotrichum truncatum, colletotrichum fructicola, alternaria solani, fusidium coccineum and rhizoctonia solani. A novel water-dispersing granular of the mutagenesis strain YL13 is researched, which not only can improve capability of resisting diseases, such as anthracnoseand root rot, but also can promote seedling growth. Test on prevention and treatment effect in forest shows that the YL13 microbial agent has excellent prevention and treatment effect on camellia oleifera anthracnose and can reach more than 80% in forest prevention and treatment effect, which is comparable with that of chemical preparations, such as chlorothalonil.

Description

technical field [0001] The invention belongs to the technical field of biological control, and relates to a preparation containing bacillus subtilis YL13 for antagonizing diseases and / or promoting plant growth and its application. Background technique [0002] At present, the control of forest diseases still adopts the method of chemical prevention and control. This method not only leads to the pollution of the ecological environment such as soil, air, and water, but also causes pesticide residues in forest by-products, and also kills natural enemies by mistake, resulting in the destruction of ecological balance; long-term application Pesticides also increase the resistance of harmful organisms; at the same time, there are certain limitations in the persistence of the effects of chemical pesticides. Therefore, it is urgent to find an economical, safe and effective control measure. Biological control has become an important way to control plant diseases due to its safety, hi...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P3/00A01P21/00C12N15/01C12N13/00C12N1/20C12R1/125
CPCA01N63/00C12N1/20C12N13/00C12N15/01
Inventor 刘君昂刘慧年谭祥丰周国英朱嘉成董文统
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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