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Plant chlorophyll determination method and extraction device

A method for measuring chlorophyll, which is applied in the field of chlorophyll detection, can solve problems such as unfavorable on-site observations, and achieve the effects of low test cost, easy operation, and maintenance of test accuracy

Active Publication Date: 2020-08-25
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the more critical issue is that spectroscopic analysis requires a specific spectrometer, which is measured in a specific environment, which is not conducive to a large number of field observations

Method used

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  • Plant chlorophyll determination method and extraction device
  • Plant chlorophyll determination method and extraction device
  • Plant chlorophyll determination method and extraction device

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Such as figure 1 Shown, the plant chlorophyll determination method, according to the chlorophyll extract liquid image determination plant chlorophyll content, comprises the following steps:

[0042] S1. Preprocessing the image of the chlorophyll extract solution;

[0043] S2. Constructing an area chain for the preprocessed image;

[0044] S3. Perform noise reduction processing on the preliminary construction of the area chain;

[0045] S4. Define the effective area, and recalculate the effective area chain according to the effective area;

[0046] S5. Load the chlorophyll extract image, and calculate the G and L values ​​in the effective area;

[0047] S6. Calculate the exposure intensity parameter, and select an empirical function to calculate the chlorophyll content according to the exposure intensity parameter.

Embodiment 2

[0049] A method for measuring plant chlorophyll, comprising the following steps:

[0050] S11. Extracting chlorophyll from leaves, obtaining a fully leached chlorophyll extraction solution, and obtaining image data of the chlorophyll extraction solution.

[0051] Extract leaf chlorophyll, obtain fully leached chlorophyll extraction solution, and obtain image data of chlorophyll extraction solution, specifically including: collecting 3-10 normal mature leaves of the plant to be tested, and using a leaf puncher 2 to punch a leaf disc with a diameter of 1mm 5 A total of 10 tablets (or 666.6mg) were placed in the chlorophyll extraction device, and 4ml of 95% ethanol (or 80% acetone or warmed dimethyl sulfide (DMSO)) was added to extract for 48 (or 24 or 4 hours) hours. RGB images were taken after the leaves were completely white, shaken, and stationary.

[0052]Use a CCD SLR, ordinary digital camera or mobile phone to shoot RGB images, and the white wall or printing paper should ...

Embodiment 3

[0065] The difference between embodiment three and embodiment two lies in the extraction of leaf chlorophyll, obtaining fully leached chlorophyll extract solution, and the specific steps of obtaining the image data of chlorophyll extract solution, and the rest are the same, including:

[0066] Collect 3-10 normal mature leaves of the plant to be tested, use the leaf puncher 2 to get a total of 10 leaf discs 5 (or 666.6 mg) with a diameter of 1 mm, and take 10 leaf discs (or 666.6 mg) mg) into a 10ml centrifuge tube 4, add 4ml of 95% ethanol (or 80% acetone or heated dimethyl sulfide (DMSO)), and extract for 48 (or 24 or 4 hours) hours. After the leaves are completely white, shake them evenly, and then pour them into the chlorophyll extraction device for RGB image shooting.

[0067] Such as Figure 4 As shown, the chlorophyll extraction device includes a number of transparent glass cuvettes 1 and blade punchers 2 arranged side by side with a length x width x height of 10mm x 1...

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Abstract

The invention discloses a plant chlorophyll determination method, which determines plant chlorophyll content according to a chlorophyll extracting solution image, and comprises the steps of pre-treating the chlorophyll extracting solution image; constructing a region chain for the pre-treated image; performing noise reduction treatment on the preliminarily constructed region chain; defining an effective area, and recalculating an effective area chain according to the effective area; loading the chlorophyll extracting solution image, and calculating G and L values in the effective area; calculating exposure intensity parameters, and selecting, according to the exposure intensity parameters, an empirical function to calculate the chlorophyll content. The invention also discloses a chlorophyll extraction device. The method and the device provided by the invention are suitable for rapid batch determination, reduce systematic errors by standard value combining determination, are not limitedby a spectrometer, are not limited by test time and space, can still observe the chlorophyll content of plant leaves without a spectrophotometer, are more suitable for the determination of mixed samples of chlorophyll and other pigments, and have the advantages of low test cost, simple method and easy operation.

Description

technical field [0001] The invention relates to the technical field of chlorophyll detection, in particular to a method for measuring plant chlorophyll, a device for extracting chlorophyll from leaves and taking RGB pictures. Background technique [0002] The determination of chlorophyll is an important index to measure the growth state of plants. People's estimation methods for plant chlorophyll have been continuously improved from qualitative estimation by naked eyes to quantitative analysis by colorimetric analysis, and from chemical analysis to spectral analysis. [0003] At present, the quantitative determination of chlorophyll content is generally carried out by spectral analysis. This method is based on the principle of measuring the light transmittance of pigments, has the advantages of convenience and reliability, and is more suitable for the determination of chlorophyll content in green plant leaves. However, for some mixed-color leaves that contain both chloroph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/25
CPCG01N21/25
Inventor 王斐宋磊王静董玉峰秦光华杜震宇
Owner SHANDONG FOREST SCI RES INST
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